IMMUNOASSAYS FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目前检测结核分枝杆菌(M.tb.)非人灵长类动物的感染有很大的局限性。因此，本项目旨在开发新的多重免疫分析方法来检测对结核分枝杆菌的抗体和细胞介导的免疫反应。在恒河猴身上。该项目建立在(1)分枝杆菌基因组学、蛋白质组学和免疫学知识以及(2)新的传染病诊断工具方面的最新进展。本项目中开发的新的多重免疫分析方法对于建立和维护非人类灵长类动物的无特定病原体(SPF)菌落至关重要，以便在生物医学研究中最佳使用，包括利用猕猴进行艾滋病研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Current methods to detect Mycobacterium tuberculosis (M.tb.) infection of non-human primates have significant limitations. Accordingly, this project aims to develop novel multiplex immunoassay methods to measure both antibody and cell-mediated immune responses to M.tb. in rhesus macaques. This project builds on recent advances in (1) knowledge of mycobacteria genomics, proteomics, and immunology and (2) novel diagnostic instrumentation for infectious disease. The novel multiplex immunoassay methods developed in this project will be essential for establishing and maintaining specific-pathogen-free (SPF) colonies of non-human primates for optimal use in biomedical research, including the use of macaques for AIDS research.