IMMUNOASSAYS FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS

检测结核分枝杆菌的免疫测定法

• 批准号: 8172553
• 负责人: Paul Luciw
• 金额: $ 7.6万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172553
• 关键词: Acquired Immunodeficiency Syndrome; Antibodies; Antigens; Biomedical Research; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Detection; Funding; Genomics; Genus Mycobacterium; Grant; Immune response; Immunoassay; Immunology; Infection; Institution; Knowledge; Macaca; Macaca mulatta; Measures; Methods; Mycobacterium tuberculosis; Proteomics; Recombinants; Research; Research Personnel; Resources; Source; Specific Pathogen Frees; System; Technology; United States National Institutes of Health; austin; base; cell mediated immune response; germ free condition; instrumentation; nonhuman primate; novel; novel diagnostics; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Current methods to detect Mycobacterium tuberculosis (M.tb.) infection of non-human primates have significant limitations. Accordingly, this project aims to develop novel multiplex immunoassay methods, based on Luminex technology (Austin, TX) to measure both antibody and cell-mediated immune responses to M.tb. in rhesus macaques. This project builds on recent advances in (1) knowledge of mycobacteria genomics, proteomics, and immunology and (2) novel diagnostic instrumentation for infectious disease. The multiplex immunoassay system detects immune responses to multiple M.tb. recombinant antigens. This serodetection method for M.tb., as well as for other infectious pathogens, will be important for establishing and maintaining specific-pathogen-free (SPF) colonies of non-human primates for optimal use in biomedical research, including the use of macaques for AIDS research.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目前检测结核分枝杆菌（M.tb.）非人灵长类动物感染具有显著的局限性。 因此，该项目旨在开发基于Luminex技术（Austin，TX）的新型多重免疫测定方法，以测量针对结核分枝杆菌的抗体和细胞介导的免疫应答。在恒河猴中。 该项目建立在（1）分枝杆菌基因组学，蛋白质组学和免疫学的知识和（2）传染病的新型诊断仪器的最新进展。 多重免疫测定系统检测对多种结核分枝杆菌的免疫应答。重组抗原 该结核分枝杆菌血清检测方法，以及其他传染性病原体的感染，对于建立和维持非人灵长类动物的无特异性病原体（SPF）菌落以最佳地用于生物医学研究，包括使用猕猴进行AIDS研究将是重要的。