MULTIPLEX IMMUNOASSAY FOR ANTIBODIES IN MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 目的：为了维持健康的非人灵长类动物用于生物医学和AIDS相关研究，在大多数灵长类动物设施中常规筛选动物的几种感染因子。在目前的实践中，通过常规免疫测定法（如酶联免疫吸附测定法（ELISA）或蛋白质印迹法）检测猴血清样品，以检测特异性抗体。为了测试每个样品中针对多种试剂的抗体，常规免疫测定是费力且耗时的。目的是开发更有效的免疫检测方法，用于非人灵长类动物的血清监测。 因此，我们开发了一种新型的多重血清学诊断系统，其中可单独识别的荧光微珠包被有纯化的全病毒抗原。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To maintain healthy nonhuman primates for use in biomedical and AIDS related research, animals are routinely screened at most primate facilities for several infectious agents. In current practice, monkey serum samples are tested by conventional immunoassays such as enzyme linked immunosorbent assay (ELISA) or Western blot for the detection of specific antibodies. For testing antibodies against multiple agents in each sample, conventional immunoassays are laborious and time consuming. The objective is to develop more efficient immunoassays for serosurveillance of nonhuman primates. Accordingly, we have developed a novel multiplex serodiagnostic system where individually identifiable, fluorescent microbeads are coated with purified whole viral antigens.