MULTIPLEX IMMUNOASSAY FOR ANTIBODIES IN MACAQUES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目的：为了保持健康的非人灵长类动物用于生物医学研究，在大多数灵长类动物设施中对动物进行常规筛查。在目前的实践中，猴子血清样本是通过传统的免疫分析方法进行测试的，例如酶联免疫吸附试验（ELISA）或Western blot来检测特异性抗体。为了在每个样品中检测针对多种试剂的抗体，传统的免疫测定既费力又耗时。目的是为非人类灵长类动物的血清监测开发更有效的免疫测定方法。因此，我们开发了一种新的多重血清诊断系统，其中单独可识别的荧光微珠被纯化的整个病毒抗原包裹。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To maintain healthy nonhuman primates for use in biomedical research, animals are routinely screened at most primate facilities for several infectious agents. In current practice, monkey serum samples are tested by conventional immunoassays such as enzyme linked immunosorbent assay (ELISA) or Western blot for the detection of specific antibodies. For testing antibodies against multiple agents in each sample, conventional immunoassays are laborious and time consuming. The objective is to develop more efficient immunoassays for serosurveillance of nonhuman primates. Accordingly, we have developed a novel multiplex serodiagnostic system where individually identifiable, fluorescent microbeads are coated with purified whole viral antigens.