NEURAL SUBSTRATES CROSS-MODAL INTEGRATION OF SOCIO-EMOTIONAL CUES: PET IMAGING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目将眼睛跟踪和PET成像技术与恒河猴的优先观看任务相结合，以评估社交显著线索跨模式整合背后的神经基础，这项技能被认为是自我调节社会行为的关键。我们已经购买了所有的设备，获得了所有的猴子面部刺激和发声，并设置了所有设备，用于记录动物被动观看一致(与面部情绪对应的发声)和不一致刺激(发声与面部情绪不对应)时的视觉搜索模式。 在两只正常成年猴子身上完成了初步测试，他们的数据重复了之前的发现，即正常发育的猴子表现出一种天生的能力，能够整合复杂社交线索的音频和视觉成分。使用这些标准，我们现在已经开始测试杏仁核和眼眶额叶皮质新生损伤的实验动物和假手术对照组。在本年度，我们还购买了眼球跟踪系统，目前正在培训6只猴子使用该范式。初步测试表明，动物很快就习惯了刺激的呈现。 在PET成像所需的25分钟测试期间，我们对刺激呈现的时间表进行了重大调整，并加入了额外的刺激以减少习惯化。第一个受试者将在来年准备进行成像试验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project combines eye-tracking and PET imaging techniques with a preferential looking task in rhesus macaques to assess the neural substrates underlying cross-modal integration of socially salient cues, a skill thought to be critical in self-regulation of social behavior. We have purchased all equipment, acquired all of the monkey face stimuli and sound vocalizations, and set-up all the equipment for the recording of the visual search patterns while the animals are passively viewing congruent (vocalization corresponding to facial emotions) and incongruent stimuli (vocalization not corresponding to facial emotions). Pilot testing has been completed on two normal adult monkeys and their data replicate previous findings such that normally developing monkeys exhibit an innate ability to integrate the audio and visual components of complex social cues. Using these criteria, we have now begun to test the experimental animals with neonatal lesions of the amygdala and orbital frontal cortex and the sham-operated controls. During the current year, we have also purchased the eye-tracking system and are currently training 6 monkeys on the paradigm. Initial tests indicate that animals rapidly habituate to stimuli presentation. We made significant adjustment in the schedule of stimuli presentation and inclusion of additional stimuli to reduce habituation during the 25 minute testing period required for PET imaging. The first subject will be ready for imaging trials during the coming year.