DEVELOPMENT OF REVERSIBLE INACTIVATION TECHNIQUE STUDY HIGHER COGNITIVE FUNCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这项研究中，我们使用最先进的可逆失活技术来更详细地探讨猴子内侧颞叶不同组成部分之间在记忆功能中的相互作用。这些方法允许在测试某些特定的记忆功能时，有选择地在短时间内关闭和打开内侧颞叶内的个别结构。这些可逆的失活过程创造了“虚拟损伤”，因为潜在的神经电路不会在结构上受到干扰，而特定的部位只是暂时失活。 在报告期间，我们对一只成年雄性猴子进行了行为训练，以测量识别记忆能力。在一半的测试过程中，目标结构被蝇草酚(GABA激动剂)灭活，而在另一半的测试过程中，则注入了赋形剂。 我们的初步数据表明，麝香酚在25分钟到1小时内达到最大的行为效果，之后这种效果开始减弱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study, we used state-of-the-art reversible inactivation techniques to probe in greater detail the interactions between different components of the medial temporal lobe in memory functions in monkeys. These methods permit individual structures within the medial temporal lobe to be selectively turned "off" and "on" for a short period of time while some specific memory functions are tested. These reversible inactivation procedures create "virtual lesions" as the underlying neural circuitry is not structurally disrupted and a particular site is only temporarily deactivated. During the reporting period, we behaviorally trained one adult male monkey to measure recognition memory abilities. In half of the testing sessions the target structure was inactivated with muscimol (GABA agonist), and with the other half, vehicle was injected. Our preliminary data indicate that muscimol achieved maximal behavioral effect between 25 minutes to one hour, after which the effects begin to wear off.