Genomics

基因组学

• 批准号: 8335549
• 负责人: MICHAEL V. SEIDEN
• 金额: $ 13.82万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-21 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8335549
• 关键词: Address; Biochemistry; Biological Assay; Biological Markers; Biotechnology; Cancer Center Support Grant; Cells; Chromosomal Duplication; Chromosome Deletion; Chromosomes; Communication; Comprehensive Cancer Center; Cost Savings; Cytogenetics; DNA; DNA Microarray Chip; DNA Sequence Analysis; DNA copy number; Detection; Diagnosis; Early Diagnosis; Economics; Epigenetic Process; Equipment; Experimental Designs; Faculty; Fox Chase Cancer Center; Foxes; Funding; Gene Expression; Genetic; Genomics; Genotype; Institution; Instruction; Label; Laboratories; Location; Microarray Analysis; Molecular Cytogenetics; Parents; Patient Care; Peer Review; Population Heterogeneity; Proteins; Proteomics; RNA; Research; Research Personnel; Research Support; Resolution; Resource Sharing; Resources; Sampling; Scanning; Services; Solutions; Specimen; Time; Tissues; Training; Translational Research; anticancer research; cancer prevention; cancer therapy; cost effectiveness; genome-wide; improved; instrumentation; laser capture microdissection; operation; programs; tumor; tumor molecular fingerprint

PROJECT SUMMARY (See instructions): The Genomics Facility (GF) was created by merging previously approved CCSG cores (Research Cytogenetics and Laser Capture Microdissection, DNA Microarray, and Cancer Prevention Biomarker and Genotyping Facilities) in order to increase cost-effectiveness and efficiency, consolidate space, increase intellectual and technical communication among staff, and provide cross-training important for laboratory stability. In addition, the Real-Time PCR service, previously part of the Biochemistry & Biotechnology Facility, was integrated into the merged GF to avoid redundancy. This merger was recommended by a Facility Parent Oversight Committee (FPOC). The FPOC is an institutional committee comprised of a Faculty Chair and mid to senior level faculty. The FPOC mandate is to prioritize CCSG funded shared resources. The GF consists of four component services: Cytogenetics & Chromosome Microarray Analysis, Laser Capture Microdissection (LCM), DNA Microarray, Genotyping and Real-time PCR. The Cytogenetics and Chromosome Microarray Analysis services performs classical cytogenetics and molecular cytogenetics, as well as high-resolution DNA copy number analysis for the detection of genomic imbalances, including chromosomal deletions and amplifications. The LCM service provides instrumentation and expertise needed for isolating pure cell populations from heterogeneous tissue specimens. The purified DNA, RNA or protein from captured cells can be used for a wide range of downstream applications such as gene expression analysis, DNA sequencing, and proteomic assays. The DNA Microarray service provides investigators with gene expression analysis solutions for cancer research. The services of this unit include microarray experimental design, RNA sample labeling, hybridization and scanning. The Genotyping and Real-time PCR service provides various platforms and expertise for genome-wide and targeted DNA and RNA analyses as well as quantitative real-time PCR services. Thirty-six peer-reviewed, funded investigators utilized the GF in CY2009. Merging the previously distinct cores has provided both operational and economic advantages. The institution has provided contiguous laboratory space for this merged operation to promote synergies such as cross training, sharing of equipment and efficiency of workflow. In addition, the merger has produced real cost savings. Unifying the operation has reduced by 1.7 the total FTE's dedicated to providing these services previously as separate cores.

项目总结（见说明）： 基因组学设施（GF）是通过合并先前批准的CCSG核心（研究细胞遗传学和激光捕获显微切割，DNA微阵列和癌症预防生物标志物和基因分型设施）创建的，以提高成本效益和效率，巩固空间，增加工作人员之间的知识和技术交流，并为实验室提供重要的交叉培训 稳定此外，实时PCR服务，以前的生物化学和生物技术设施的一部分，被整合到合并后的GF，以避免冗余。这一合并是由设施母公司监督委员会（FPOC）建议的。FPOC是一个机构委员会，由一个教师主席和中高级教师组成。FPOC的任务是优先考虑CCSG资助的共享资源。GF由四个组成部分组成：细胞遗传学和染色体微阵列分析， 激光捕获显微切割（LCM），DNA微阵列，基因分型和实时PCR。细胞遗传学和染色体微阵列分析服务进行经典的细胞遗传学和分子细胞遗传学，以及高分辨率DNA拷贝数分析，用于检测基因组不平衡，包括染色体缺失和扩增。LCM服务提供所需的仪器和专业知识 用于从异质组织样本中分离纯细胞群。从捕获的细胞中纯化的DNA、RNA或蛋白质可用于广泛的下游应用，例如基因表达分析、DNA测序和蛋白质组学测定。DNA微阵列服务为癌症研究人员提供基因表达分析解决方案。该单位的服务包括微阵列 实验设计、RNA样品标记、杂交和扫描。基因分型和实时PCR服务为全基因组和靶向DNA和RNA分析以及定量实时PCR服务提供各种平台和专业知识。2009年，36名经同行评审、获得资助的研究者使用了GF。合并以前不同的核心提供了操作和经济优势。 该机构为合并后的业务提供了连续的实验室空间，以促进协同效应，例如交叉培训，共享设备和工作流程的效率。此外，合并产生了真实的成本节约。统一操作使以前作为单独核心提供这些服务的FTE总数减少了1.7。