DNA Facility

DNA设施

• 批准号: 8335539
• 负责人: MICHAEL V. SEIDEN
• 金额: $ 3.26万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-21 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8335539
• 关键词: Advisory Committees; Archives; Biomedical Research; Blood capillaries; Calendar; Cells; Communities; Comprehensive Cancer Center; Computer software; Computers; DNA; DNA Sequence; DNA Sequencing Facility; Data; Development; Fox Chase Cancer Center; Foxes; Funding; Host Defense; Hour; Immune; Instruction; Laboratory Research; Licensing; Modeling; Mutation; Parents; Peer Review; Preparation; Reaction; Recommendation; Research; Research Personnel; Running; Sampling; Sequence Analysis; Site; Structure; Technology; base; capillary; cost effective; gene cloning; instrument; programs; tool; vector

PROJECT SUMMARY (See instructions): The DNA Sequencing Facility (DSF) provides investigators with high quality DNA sequencing. In calendar year 2009, the Facility was used by 43 peer-reviewed, funded investigators across four of the Center's five Research Programs. Ninety-eight and one-half percent (98.5%) of use is in support of peer-reviewed, funded investigators. Hardy (Immune Cell Development and Host Defense (ICDHD) Program) assumed direction of the Facility in 1993. In 2004 all automated sequencers were replaced by a single ABI 3100 capillary instrument, providing more reliable high-throughput sequencing. The ABI 3100 was upgraded to a model 3130 in 2007. The DSF provides Fox Chase Cancer Center (FCCC) investigators with computer-readable sequences of their DNA samples in a timely and cost effective manner. Automated sequence analysis is used by investigators to verify DNA constructs, to identify mutations, and to determine the structure of newly cloned genes. The volume of usage of the Facility has continued at a high level averaging 16,000 sequences per year over the last five years. Equitable access and operational oversight of the Facility are assured by recommendations from a Facility Advisory Committee (FAC) and the Facility Parent Oversight Committee (FPOC).

项目摘要（请参阅说明）： DNA测序设施（DSF）为研究人员提供了高质量的DNA测序。在2009年日历年中，该设施在该中心的五个研究计划中的四个经过同行评审的研究人员中使用了该设施。使用的98％和一半（98.5％）用于支持经过同行评审的资助的研究人员。 Hardy（免疫细胞开发和宿主防御（ICDHD）计划）在1993年假设了该设施的方向。2004年，所有自动序列均由A取代 单个ABI 3100毛细管仪器，提供更可靠的高通量测序。 ABI 3100于2007年升级为3130型。DSF为Fox Chase Cancer Center（FCCC）研究人员提供了及时有效的DNA样品的计算机可读序列。研究人员使用自动序列分析来验证DNA构建体，识别突变并确定 新克隆基因的结构。在过去五年中，该设施的使用量持续了高水平，平均每年16,000个序列。设施咨询委员会（FAC）和设施父母监督委员会（FPOC）的建议确保了对设施的公平访问和运营监督。