Histopathology

项目总结（见说明）： 组织学设施（HF）的目的是通过提供组织学和病理学服务，包括细胞和组织的处理以及帮助解释结果，促进福克斯蔡斯癌症中心（FCCC）资助的研究人员进行的研究。该机构的技术人员和病理学家在该中心的许多研究项目中发挥着重要的支持作用，他们的贡献一直并将继续在动物模型的建立和表征方面发挥重要作用。 人类癌症的模型。该设施准备，处理，并协助评估来自同行评审，资助研究人员开发的实验方案的组织。大多数项目涉及人类癌症的动物模型，需要复杂的组织学和/或细胞学处理来确定形态学改变，以及定位癌症相关的基因产物。2005 - 2009年期间，该机制最经常使用的服务包括：实验动物尸检、石蜡包埋组织块的固定、包埋和切片（每年8，000 - 12，000例），用于免疫组织化学（IHC）和激光捕获显微切割（LCM）的未染色石蜡切片（每年9，000 - 14，000例），冷冻切片术（每年200 - 1，500），特殊污渍（每年200 - 500例），免疫组织化学（IHC）（每年2，100 - 3，700），数字显微摄影（每年3，000 - 5，000）和解释性组织病理学（每年7，000 - 10，000 H & E和IHC切片）。在上一个CCSG资助周期，我们看到对所有服务的稳定需求和执行的冷冻切片数量的显著增加（100%）。此外，在2005 - 2010年期间，我们巩固了新技术，如基于网络的实验历史学数据库，并引入了计算机图像分析。HF还在质量控制和动物健康监测活动方面为实验室动物设施（LAF）提供支持，并为生物样品储存设施（BRF）和基因组学设施（GF）的LCM组件提供处理支持。2009年，在FCCC的所有五个研究项目中，有39名同行评审、资助的研究者使用了HF。

PROJECT SUMMARY (See instructions): The' purpose of the Histopathology Facility (HF) is to facilitate research conducted by funded investigators at Fox Chase Cancer Center (FCCC) by providing histology and pathology services including processing of cells and tissues as well as helping in the interpretation of results. The Facility's Technicians and Pathologist play a major support role in numerous research projects at the Center and their contributions have been, and continue to be of great significance in the establishment and characterization of animal models of human cancer. The Facility prepares, processes, and assists in evaluating tissues derived from experimental protocols developed by peer-reviewed, funded investigators. Most of the projects involve animal models of human cancer that require complex histological and/or cytological processing to determine morphological alterations, as well as to localize cancer-relevant gene products. The most frequently utilized services of the Facility during 2005-2009 include: laboratory animal autopsy, fixation, embedding and sectioning of paraffin-embedded tissue blocks (8,000-12,000 per year), unstained paraffin sections for immunohistochemistry (IHC) and laser capture microdissection (LCM) (9,000-14,000 per year), cryomicrotomy (200-1,500 per year), special stains (200-500 per year), immunohistochemistry (IHC) (2,100-3,700 per year), digital microphotography (3,000-5,000 per year), and interpretative histopathology (7,000- 10,000 H&E and IHC slides signed-out per year). During the last CCSG funding cycle we have seen a steady need for all services rendered and significant increase (100%) in the number of frozen sections performed. Furthermore, during 2005-2010 we consolidated new technology such as the web-based Experimental Histopathology database and introduced computerized image analysis. The HF also provides support to the Laboratory Animal Facility (LAF) in quality control and animal health monitoring activities, as well as processing support to the Biosample Repository Facility (BRF) and the LCM component of the Genomics Facility (GF). The HF was used by 39 peer-reviewed, funded investigators in all five Research Programs at FCCC, in calendar year 2009.