Infrared microscopy for a systems approach to prostate pathology

用于前列腺病理学系统方法的红外显微镜

• 批准号: 8215876
• 负责人: Rohit Bhargava
• 金额: $ 31.27万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8215876
• 关键词: Address; Age; Aging; Animal Model; Automation; Biochemical; Biology; Biopsy; Cataloging; Catalogs; Cessation of life; Characteristics; Chemicals; Clinical; Clinical Data; Clinical Research; Collaborations; Computational algorithm; Data Analyses; Decision Aid; Diagnosis; Disease; Dyes; Economics; Engineering; Epidemic; Epithelial Cells; Expenditure; Family; Fourier Transform; Gleason Grade for Prostate Cancer; Goals; Gold; Healthcare; Histologic; Histology; Histopathology; Human; Image; Imaging technology; Incidence; Indolent; Knowledge; Label; Laboratories; Lead; Lesion; Malignant Neoplasms; Malignant neoplasm of prostate; Manuals; Measurement; Measures; Methods; Modeling; Molecular; Morphology; Nomograms; Outcome; Pathologic; Pathologist; Pathology; Patients; Pattern; Persons; Pharmaceutical Preparations; Population; Prostate; Quality of life; Reagent; Research; Resolution; Resources; Risk; Risk Factors; Route; Sampling; Scientist; Spectrum Analysis; Surgeon; System; Systems Analysis; Technology; Time; Tissue Stains; Tissues; Training; Translating; Triage; Workload; analytical method; anticancer research; base; cell type; cellular development; clinical care; cohort; computerized; improved; infrared microscopy; insight; instrument; instrumentation; men; new technology; novel; novel strategies; operation; prognostic; public health relevance; response; spectroscopic imaging; tumor

DESCRIPTION (provided by applicant): Technologies that can provide new prognostic information and/or help make accurate, objective and automated decisions that aid the pathologist are sorely needed in prostate healthcare. The major goal of this project is to provide a chemical imaging instrument for clinical and research use that can be operated by any trained person in pathology laboratories. First, novel instrumentation and analytical methods for high-resolution Fourier transform infrared (FT-IR) spectroscopic imaging will be developed. Second, both structural (morphologic) and biochemical transformations that are characteristic of slow and rapidly growing tumors will be quantified. Third, analytical methods will be developed to relate the extracted information to aggressive potential of the tumor. Predictions will be compared to the clinical standard today (Kattan nomogram). A systems analysis approach is proposed to examine important histologic and chemical transformations that characterize aggressive lesions. New biochemical insight is expected from these models that will lead ultimately to an explanation of why we are able to define aggressive lesions accurately. If the project is successful, it has the potential to transform therapy decisions for patients and alter the standard practice in histologic assessment of research. PUBLIC HEALTH RELEVANCE: The major goal of this project is to leverage novel chemical imaging technology to identify those truly at risk of death from prostate cancer. To achieve this goal, we develop novel chemical imaging technology that can measure the spatial and molecular content of tissue as without the use of dyes or labels. Analytical methods will be developed to extract information from the measurements and relate it to biochemical knowledge of the prostate. A systems pathology approach, finally, is presented to predict those at risk. The project has the potential to transform therapy decisions for patients by identifying aggressive and indolent lesions accurately. This would enable focusing of resources on ~40,000 patients who have lethal disease and avoid wasteful expenditures on ~200,000 people. If successful, establishment of the instrumentation and analytical methods here would alter the standard practice in clinical histologic assessment of research in prostate cancer.

描述（由申请人提供）：前列腺保健领域迫切需要能够提供新的预后信息和/或帮助病理学家做出准确、客观和自动化决策的技术。该项目的主要目标是提供一种供临床和研究使用的化学成像仪器，可由病理实验室中任何经过培训的人员操作。首先，将开发用于高分辨率傅里叶变换红外（FT-IR）光谱成像的新型仪器和分析方法。其次，将量化缓慢和快速生长的肿瘤特征的结构（形态）和生化转变。第三，将开发分析方法将提取的信息与肿瘤的侵袭潜力联系起来。预测将与当今的临床标准（Kattan 列线图）进行比较。提出了一种系统分析方法来检查表征侵袭性病变的重要组织学和化学转变。预计这些模型将带来新的生化见解，最终将解释为什么我们能够准确定义侵袭性病变。如果该项目成功，它有可能改变患者的治疗决策，并改变研究组织学评估的标准实践。 公共健康相关性：该项目的主要目标是利用新型化学成像技术来识别那些真正面临前列腺癌死亡风险的人。为了实现这一目标，我们开发了新型化学成像技术，无需使用染料或标签即可测量组织的空间和分子含量。将开发分析方法从测量中提取信息并将其与前列腺的生化知识联系起来。最后，提出了一种系统病理学方法来预测处于危险中的人。该项目有可能通过准确识别侵袭性和惰性病变来改变患者的治疗决策。这将使资源能够集中用于约 40,000 名患有致命疾病的患者，并避免在约 200,000 人身上浪费开支。如果成功，这里仪器和分析方法的建立将改变前列腺癌研究的临床组织学评估的标准实践。