The Ivis Spectrum CT for Pre-Clinical Oncology Research

描述(申请人提供)：申请资金购买Ivis Spectrum CT光学和计算机断层扫描(CT)成像系统(卡利珀生命科学，马萨诸塞州霍普金顿)用于体内定量生物光子(即生物发光和荧光)成像，用于非侵入性和纵向监测(A)癌症和其他疾病过程，(B)癌症、免疫和干细胞的传播，(C)细胞对治疗和其他干预措施的反应，以及(D)小动物(即小鼠和大鼠)肿瘤模型中的遗传途径表达。与我们现有的两个IVIS系统IVIS 100和200一样，这款最先进的仪器将安装在我们的小动物成像核心设施中，它将是纪念斯隆-凯特琳癌症中心(MSKCC)临床前和转化性肿瘤学研究计划中广泛使用的关键组件。活体生物发光和荧光成像已经迅速成为世界范围内临床前生物医学研究的主要内容。然而，我们的Ivis 100和200系统的使用量非常大(现在已经超出了容量)，而且它们的使用量还在继续增加--它们是迄今为止使用量最大的小型动物成像仪器，支持着整个MSKCC多达30多个实验室。因此，不存在备份/冗余。因此，当这些系统中的一个需要维修时，实验工作就会被推迟，可能会受到影响，甚至会丢失。此外，Ivis 100和200现在分别使用了9年和6年，接近或已经超过了它们的使用寿命，因此随时都会出现无法修复的故障。除了提供急需的额外成像能力和备份外，Ivis Spectrum CT还将提供关键的新功能，包括(1)光学和近红外(NIR)范围的透照荧光成像，提供更高的光谱分辨率和荧光成像对比度；(2)三维(3D)/断层成像能力，可提供关键深度信息；以及(3)基于CT的集成三维成像，可实现光学图像和解剖图像的无缝、近乎完美的配准，从而对光学信号焦点在体内进行明确的定位。我们的设施和中心拥有成熟的技术和科学专业知识，可以充分和立即地利用这一仪器，并确保其安装和有效的行政和财务基础设施，以支持和维护这一仪器及其长期有效使用。

DESCRIPTION (provided by applicant): Funds are requested to purchase an Ivis Spectrum CT optical and computed tomography (CT) imaging system (Caliper Life Sciences, Hopkinton, MA) for quantitative in vivo bio photonic (i.e. bioluminescent and fluorescent) imaging for non-invasively and longitudinally monitoring (a) cancer and other disease processes, (b) trafficking of cancer, immune, and stem cells, (c) cellular responses to therapy and other interventions, and (d) genetic pathway expression in small-animal (i.e. mouse and rat) tumor models. Like our two existing Ivis systems, the Ivis 100 and 200, this state-of-the-art instrument, to be installed n our Small-Animal Imaging Core Facility, will be a critical, broadly used component of the pre-clinical and translational oncology research program at Memorial Sloan-Kettering Cancer Center (MSKCC). In vivo bioluminescent and fluorescent imaging has rapidly become a staple of pre-clinical biomedical research world-wide. However, both our Ivis 100 and 200 systems are used very heavily (and, now beyond capacity) and their use continues to increase - they are by far the most heavily used of our small-animal imaging instruments, supporting up to three dozen laboratories throughout MSKCC. Thus, there is no back-up/redundancy. When one of these systems requires service, therefore, experimental work is delayed and potentially compromised and even lost. Further, the Ivis 100 and 200 are now 9- and 6-years-old, respectively, and approaching or already beyond their useful lifespan and thus subject to irreparable failure at any moment. Besides providing urgently needed additional imaging capacity and back-up, the Ivis Spectrum CT would provide critical new functionality, including (1) trans-illumination fluorescence imaging in both the optical and near-infrared (NIR) range, providing far better spectral resolution and fluorescence-imaging contrast; (2) three-dimensional (3D)/ tomographic imaging capability to provide critical depth information; and (3) integrated CT-based 3D imaging to allow seamless near-perfect registration of optical and anatomic images and thereby unambiguous localization in vivo of optical signal foci. Our Facility and Center have well-established technical and scientific expertise to fully and immediately utilize this instrument upo its installation and an efficient administrative and financial infrastructure to support and maintan this instrument and its effective use long-term.