The Oral Microbiome in HIV-associated Oral Warts and Candidiasis

HIV 相关口腔疣和念珠菌病中的口腔微生物组

• 批准号: 9331335
• 负责人: PAUL L FIDEL
• 金额: $ 54.12万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-21 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9331335
• 关键词: Affect; Age; Anti-Retroviral Agents; Bacteria; CD4 Lymphocyte Count; CD4 Positive T Lymphocytes; Candida; Candida albicans; Candidiasis; Cell Count; Clinical Research; Communities; Contracts; Cross-Sectional Studies; Data; Death Rate; Dental caries; Diabetes Mellitus; Diagnostic; Disease; Ecology; Etiology; Functional disorder; Genetic; Goals; HIV; HIV Infections; HIV antiretroviral; HIV therapy; Head and Neck Cancer; Head and Neck Squamous Cell Carcinoma; Health; Health Status; Heart Diseases; Human Papillomavirus; Immunosuppression; Individual; Infection; Lead; Left; Lipids; Manipulative Therapies; Massive Parallel Sequencing; Methodology; Mouth Diseases; Mucous Membrane; Opportunistic Infections; Oral; Oral Manifestations; Oral candidiasis; Oral cavity; Oral health; Pathogenesis; Patients; Periodontal Diseases; Play; Predisposition; Prevalence; Prognostic Marker; Race; Reporting; Risk; Risk Factors; Role; Salivary Glands; Sequence Analysis; Site; Smoking and Health Research; Syndrome; Technology; Testing; antiretroviral therapy; base; cost; expectation; falls; frontier; high risk; innovation; malignant mouth neoplasm; microbial; microbial community; microbiome; microbiota; novel; oral microbiome; oral wart; oropharyngeal thrush; pathogen; prevent; prospective; public health relevance; pyrosequencing; rRNA Genes; sex; therapeutic target; therapy development; tool

DESCRIPTION (provided by applicant): Human immunodeficiency virus (HIV) has infected over 30 million individuals worldwide. Left untreated, over 50% of these individuals will incur an oral manifestation associated with HIV. Currently, the most common oral manifestations of HIV disease are oropharyngeal candidiasis (OPC) and oral warts. In addition, HPV-associated head and neck squamous cell carcinomas (HNSCC) are increased in HIV+ individuals and have not seen a reduction in prevalence with the advent of anti-retroviral therapy (ART). The factors which determine an HIV+ individual's predisposition to OPC, HPV and other oral manifestations are poorly understood as well as how treatments for HIV influence the oral cavity and/or infections/diseases. A realistic description of the composition and diversity of the oral microflora (microbiome) is essential to understanding health and disease in the oral cavity. However, how the oral microbiome is impacted by HIV has not been described, but based on preliminary data, is expected to be significant. Moreover, ART used to treat HIV also likely impacts/alters the oral microbiome. We will use PCR-amplification of 16S rRNA genes and massive parallel sequencing to test the hypothesis that both HIV infection and ART alter the composition and diversity of bacterial species in the oral cavity and such composition can be associated with risk of oral manifestations such as HIV-associated OPC and ART-associated oral warts and HNSCC.

描述（由申请人提供）： 人类免疫缺陷病毒（HIV）已感染全世界3000多万人。如果不进行治疗，超过50%的这些人将引起与艾滋病毒相关的口腔表现。目前，HIV疾病最常见的口腔表现是口咽念珠菌病（OPC）和口腔疣。此外，HPV相关的头颈部鳞状细胞癌（HNSCC）在HIV+个体中增加，并且随着抗逆转录病毒治疗（ART）的出现，患病率并未降低。决定HIV+个体对OPC，HPV和其他口腔表现的易感性的因素以及HIV治疗如何影响口腔和/或感染/疾病知之甚少。一个现实的描述 口腔微生物区系（微生物组）的组成和多样性对于理解口腔中的健康和疾病是必不可少的。然而，尚未描述艾滋病毒如何影响口腔微生物组，但基于初步数据，预计这一影响很大。此外，用于治疗艾滋病毒的ART也可能影响/改变口腔微生物组。我们将使用16 S rRNA基因的PCR扩增和大规模平行测序来检验HIV感染和ART改变口腔中细菌种类的组成和多样性的假设，并且这种组成可能与口腔表现的风险相关，例如HIV相关的OPC和ART相关的口腔疣和HNSCC。