Single Cell Spatial Transcriptomics Shared Instrument at the BCM Core Facility

BCM 核心设施的单细胞空间转录组学共享仪器

• 批准号: 10414324
• 负责人: RUI CHEN
• 金额: $ 30.4万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414324
• 关键词: Address; Basic Science; Cells; Clinical Research; Core Facility; Disease; Ensure; Fluorescent in Situ Hybridization; Funding; Genomics; Heterogeneity; Image; In Situ; Institutes; Medical; Methods; National Center for Advancing Translational Sciences; National Heart, Lung, and Blood Institute; National Human Genome Research Institute; National Institute of Allergy and Infectious Disease; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Environmental Health Sciences; National Institute of General Medical Sciences; National Institute of Neurological Disorders and Stroke; RNA; Research; Research Project Grants; Resolution; Scheme; Technology; Tissues; United States National Institutes of Health; cell type; instrument; operation; single molecule; transcriptome; transcriptomics

Abstract Single-cell omics is a rapidly growing new field that provides many advantages over traditional ‘bulk’ tissue profiling methods, such as the ability to resolve intratissue heterogeneity in cell types, profile disease microenvironments, and study rare subpopulations. However, one of the major drawbacks of current single-cell transcriptome technologies is that they require the isolation of single cells, resulting in the loss of spatial information. To address this issue, spatial single-cell transcriptomics technologies have been recently developed, which will enable many new research opportunities. The requested instrument, the MERSCOPE from Vizgen, is currently the only commercial platform for the recently developed multiplexed error-robust fluorescence in situ hybridization (MERFISH) technology that allows in situ transcriptome profiling at single cell resolution. By utilizing error-robust and error-correcting encoding schemes, MERFISH enables massively multiplexed single-molecule RNA imaging with high sensitivity and accuracy. The shared MERSCOPE instrument will be placed at and administrated by the established BCM Single Cell Genomics Core (SCGC) to ensure smooth operation and easy access. We have identified 16 research groups at BCM as users. Together, these groups have 41 active research projects funded by the NIH and will use 90% of the capacity of the instrument. The research focuses of these projects are highly diverse, and they are funded by fifteen institutes at the NIH, including the NICHD, NIAMS, NIDC, NCATS, NIDDK, NEI, NCI, NIA, NIGMS, NHLBI, NHGRI, NINDS, NIAID, NIEHS, and NIMDH. As the participating PIs are all engaged in both basic and clinical research, we believe the introduction of this new instrument will have direct, broad, and immediate impact on many medically relevant fields.

摘要 单细胞组学是一个快速发展的新领域，它提供了许多优于传统“散装”组织的优点 概况分析方法，例如解决细胞类型、概况疾病的能力 微环境，并研究稀有亚群。然而，目前单电池的主要缺点之一是 转录组技术是，它们需要分离单个细胞，导致空间丢失 信息。为了解决这个问题，空间单细胞转录转录技术最近已经出现 这将带来许多新的研究机会。所要求的仪器，MERSCOPE 来自Vizgen，是目前唯一用于最近开发的多路复用器的商业平台-鲁棒 允许单细胞原位转录组谱分析的荧光原位杂交(MerFish)技术 决议。通过利用差错稳健和纠错编码方案，MerFish能够大规模地 具有高灵敏度和准确性的多路复用单分子RNA成像。共享MERSCOPE 仪器将放置在已建立的BCM单细胞基因组核心(SCGC)并由其管理，以 确保运行顺畅，方便取用。我们已经在BCM确定了16个研究小组作为用户。 这些小组总共有41个由NIH资助的正在进行的研究项目，并将使用90%的能力 乐器。这些项目的研究重点高度多样化，由15个国家资助 NIH的研究所，包括NICHD、NIAMS、NIDC、NCATS、NIDDK、NEI、NCI、NIA、NIGMS、NHLBI、 NHGRI、NINDS、NIAID、NIEHS和NIMDH。因为参与的PI都从事基础和临床工作 我们相信，这种新工具的引入将对以下方面产生直接、广泛和直接的影响 许多与医学相关的领域。