Single Cell Spatial Transcriptomics Shared Instrument at the BCM Core Facility

BCM 核心设施的单细胞空间转录组学共享仪器

• 批准号: 10414324
• 负责人: RUI CHEN
• 金额: $ 30.4万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414324
• 关键词: Address; Basic Science; Cells; Clinical Research; Core Facility; Disease; Ensure; Fluorescent in Situ Hybridization; Funding; Genomics; Heterogeneity; Image; In Situ; Institutes; Medical; Methods; National Center for Advancing Translational Sciences; National Heart, Lung, and Blood Institute; National Human Genome Research Institute; National Institute of Allergy and Infectious Disease; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Environmental Health Sciences; National Institute of General Medical Sciences; National Institute of Neurological Disorders and Stroke; RNA; Research; Research Project Grants; Resolution; Scheme; Technology; Tissues; United States National Institutes of Health; cell type; instrument; operation; single molecule; transcriptome; transcriptomics

Abstract Single-cell omics is a rapidly growing new field that provides many advantages over traditional ‘bulk’ tissue profiling methods, such as the ability to resolve intratissue heterogeneity in cell types, profile disease microenvironments, and study rare subpopulations. However, one of the major drawbacks of current single-cell transcriptome technologies is that they require the isolation of single cells, resulting in the loss of spatial information. To address this issue, spatial single-cell transcriptomics technologies have been recently developed, which will enable many new research opportunities. The requested instrument, the MERSCOPE from Vizgen, is currently the only commercial platform for the recently developed multiplexed error-robust fluorescence in situ hybridization (MERFISH) technology that allows in situ transcriptome profiling at single cell resolution. By utilizing error-robust and error-correcting encoding schemes, MERFISH enables massively multiplexed single-molecule RNA imaging with high sensitivity and accuracy. The shared MERSCOPE instrument will be placed at and administrated by the established BCM Single Cell Genomics Core (SCGC) to ensure smooth operation and easy access. We have identified 16 research groups at BCM as users. Together, these groups have 41 active research projects funded by the NIH and will use 90% of the capacity of the instrument. The research focuses of these projects are highly diverse, and they are funded by fifteen institutes at the NIH, including the NICHD, NIAMS, NIDC, NCATS, NIDDK, NEI, NCI, NIA, NIGMS, NHLBI, NHGRI, NINDS, NIAID, NIEHS, and NIMDH. As the participating PIs are all engaged in both basic and clinical research, we believe the introduction of this new instrument will have direct, broad, and immediate impact on many medically relevant fields.

抽象的 单细胞组学是一个快速发展的新领域，与传统的“块状”组织相比具有许多优势 分析方法，例如解决细胞类型的组织内异质性的能力、分析疾病 微环境，并研究稀有亚群。然而，当前单电池的主要缺点之一 转录组技术的缺点是它们需要分离单个细胞，从而导致空间损失 信息。为了解决这个问题，空间单细胞转录组学技术最近得到了发展。 的发展，这将带来许多新的研究机会。所需仪器 MERSCOPE 来自 Vizgen 的，是目前最新开发的多路复用抗误码技术的唯一商业平台 荧光原位杂交 (MERFISH) 技术可对单细胞进行原位转录组分析 解决。通过利用容错和纠错编码方案，MERFISH 能够大规模实现 具有高灵敏度和准确性的多重单分子 RNA 成像。共享MERSCOPE 仪器将放置在已建立的 BCM 单细胞基因组学核心 (SCGC) 并由其管理，以 确保平稳运行和轻松访问。我们已将 BCM 的 16 个研究小组确定为用户。 这些小组共有 41 个由 NIH 资助的活跃研究项目，并将使用 NIH 90% 的能力 仪器。这些项目的研究重点高度多样化，并由 15 家机构资助。 NIH 的研究所，包括 NICHD、NIAMS、NIDC、NCATS、NIDDK、NEI、NCI、NIA、NIGMS、NHLBI、 NHGRI、NINDS、NIAID、NIEHS 和 NIMDH。由于参与的PI均从事基础和临床工作 研究，我们相信这一新工具的推出将对 许多医学相关领域。