Single Cell Spatial Transcriptomics Shared Instrument at the BCM Core Facility

BCM 核心设施的单细胞空间转录组学共享仪器

• 批准号: 10414324
• 负责人: RUI CHEN
• 金额: $ 30.4万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414324
• 关键词: Address; Basic Science; Cells; Clinical Research; Core Facility; Disease; Ensure; Fluorescent in Situ Hybridization; Funding; Genomics; Heterogeneity; Image; In Situ; Institutes; Medical; Methods; National Center for Advancing Translational Sciences; National Heart, Lung, and Blood Institute; National Human Genome Research Institute; National Institute of Allergy and Infectious Disease; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Environmental Health Sciences; National Institute of General Medical Sciences; National Institute of Neurological Disorders and Stroke; RNA; Research; Research Project Grants; Resolution; Scheme; Technology; Tissues; United States National Institutes of Health; cell type; instrument; operation; single molecule; transcriptome; transcriptomics

Abstract Single-cell omics is a rapidly growing new field that provides many advantages over traditional ‘bulk’ tissue profiling methods, such as the ability to resolve intratissue heterogeneity in cell types, profile disease microenvironments, and study rare subpopulations. However, one of the major drawbacks of current single-cell transcriptome technologies is that they require the isolation of single cells, resulting in the loss of spatial information. To address this issue, spatial single-cell transcriptomics technologies have been recently developed, which will enable many new research opportunities. The requested instrument, the MERSCOPE from Vizgen, is currently the only commercial platform for the recently developed multiplexed error-robust fluorescence in situ hybridization (MERFISH) technology that allows in situ transcriptome profiling at single cell resolution. By utilizing error-robust and error-correcting encoding schemes, MERFISH enables massively multiplexed single-molecule RNA imaging with high sensitivity and accuracy. The shared MERSCOPE instrument will be placed at and administrated by the established BCM Single Cell Genomics Core (SCGC) to ensure smooth operation and easy access. We have identified 16 research groups at BCM as users. Together, these groups have 41 active research projects funded by the NIH and will use 90% of the capacity of the instrument. The research focuses of these projects are highly diverse, and they are funded by fifteen institutes at the NIH, including the NICHD, NIAMS, NIDC, NCATS, NIDDK, NEI, NCI, NIA, NIGMS, NHLBI, NHGRI, NINDS, NIAID, NIEHS, and NIMDH. As the participating PIs are all engaged in both basic and clinical research, we believe the introduction of this new instrument will have direct, broad, and immediate impact on many medically relevant fields.

摘要