Continued support for an SPF Macaca nemestrina breeding colony to provide animals to NIH funded HIV research projects

摘要 该项目的目标是提供健康、行为正常、MHC 特征、SPF 猪尾 猕猴用于美国国立卫生研究院资助的艾滋病毒/艾滋病相关研究。我们将扩大目前SPF养殖规模 群体增加用于繁殖的雌性数量以及可供出售的幼体雄性和雌性 美国国立卫生研究院 (NIH) 资助约翰·霍普金斯大学和其他研究机构的艾滋病毒/艾滋病研究人员。 保存完整的谱系和健康数据，并在动物被饲养时向研究人员提供 选定出售。病毒筛查包括对 4 种病毒因子进行定期血清学和 PCR 检测： 疱疹病毒 1（B 病毒）、猿猴免疫缺陷病毒 (SIV)、猿猴逆转录病毒 (SRV) 和猿猴 T- 淋巴营养病毒（STLV）。在过去的十年里，该群体的检测结果始终呈阴性 对于这些代理。在第一年对所有幼体进行主要组织相容性 (MHC) 分析 生命并在选择购买动物时提供给调查人员。在过去的五年里， 该群体平均每年产下 51 只后代，平均出售 32 只动物。我们 据估计，每年出售 42 只动物将使该群体实现自给自足，这是我们预计的目标 我们将在 2028 年底实现这一目标。

Abstract The aims of this project are to provide healthy, behaviorally normal, MHC-characterized, SPF pig-tailed macaques for NIH-funded HIV/AIDS related research. We will expand the size of the current SPF breeding colony to increase the numbers of females for breeding and juvenile males and females available for sale to NIH-funded HIV/AIDS investigators both within Johns Hopkins University and at other research institutions. Complete pedigrees and health data are maintained and provided to investigators when animals are being selected for sale. Viral screening includes regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV), and Simian T- lymphotrophic Virus (STLV). For the past decade the colony has consistently and repeatedly tested negative for these agents. Major histocompatibility (MHC) profiles are performed on all juveniles during the first year of life and are provided to investigators when animals are being selected for purchase. Over the last five years, the colony has produced an average of 51 offspring each year, resulting in an average of 32 animals sold. We estimate that selling 42 animals per year would allow the colony to achieve self-sufficiency, a goal we project we will reach by the end of 2028.