Continued support for an SPF Macaca nemestrina breeding colony to provide animals to NIH funded HIV research projects

继续支持 SPF 猕猴繁殖群，为 NIH 资助的艾滋病毒研究项目提供动物

• 批准号: 10576951
• 负责人: Eric Kenneth Hutchinson
• 金额: $ 101.84万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10576951
• 关键词: Acquired Immunodeficiency Syndrome; Adolescent; Animal Sources; Animals; Area; Behavioral; Biological Assay; Breeding; Cercopithecine Herpesvirus 1; Country; Disease; Female; Funding; Genome; Genomic approach; Genotype; Goals; Grant; HIV; HIV/AIDS; Histocompatibility; Institution; Life; Macaca; Macaca nemestrina; Major Histocompatibility Complex; Modeling; Population; Primates; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; SIV; Sales; Serology; Simian Retroviruses; Testing; U-Series Cooperative Agreements; United States; United States National Institutes of Health; Universities; Viral; Virus; animal breeding; deep sequencing; functional genomics; genetic pedigree; germ free condition; health data; improved; interest; male; offspring; operation; preservation; screening

Abstract The aims of this project are to provide healthy, behaviorally normal, MHC-characterized, SPF pig-tailed macaques for NIH-funded HIV/AIDS related research. We will expand the size of the current SPF breeding colony to increase the numbers of females for breeding and juvenile males and females available for sale to NIH-funded HIV/AIDS investigators both within Johns Hopkins University and at other research institutions. Complete pedigrees and health data are maintained and provided to investigators when animals are being selected for sale. Viral screening includes regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV), and Simian T- lymphotrophic Virus (STLV). For the past decade the colony has consistently and repeatedly tested negative for these agents. Major histocompatibility (MHC) profiles are performed on all juveniles during the first year of life and are provided to investigators when animals are being selected for purchase. Over the last five years, the colony has produced an average of 51 offspring each year, resulting in an average of 32 animals sold. We estimate that selling 42 animals per year would allow the colony to achieve self-sufficiency, a goal we project we will reach by the end of 2028.

摘要 该项目的目的是提供健康，行为正常，MHC特征，SPF猪尾 猕猴用于NIH资助的艾滋病毒/艾滋病相关研究。我们将扩大目前SPF育种的规模 以增加繁殖用的雌性和可供销售的幼年雄性和雌性的数量， 约翰霍普金斯大学和其他研究机构的NIH资助的艾滋病毒/艾滋病研究人员。 保存完整的谱系和健康数据，并在动物进行研究时提供给研究者。 选择销售。病毒筛查包括4种病毒因子的定期血清学和PCR检测： 疱疹病毒1（B病毒）、猿猴免疫缺陷病毒（SIV）、猿猴逆转录病毒（SRV）和猿猴T- 嗜淋巴细胞病毒（STLV）。在过去的十年里，殖民地的检测结果一直是阴性的， 对于这些代理商。主要组织相容性（MHC）的配置文件进行所有青少年在第一年的 当选择购买动物时，向研究者提供。在过去五年中， 殖民地每年平均产下51只后代，平均出售32只。我们 我估计，每年出售42只动物将使殖民地实现自给自足，这是我们计划的目标 我们将在2028年年底达到。