Viral Testing Core

病毒检测核心

• 批准号: 10403108
• 负责人: Eric Kenneth Hutchinson
• 金额: $ 11.58万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10403108
• 关键词: AIDS/HIV problem; Animals; Biological Assay; Biomedical Research; Breeding; Cercopithecine Herpesvirus 1; Communities; Development; Funding; Goals; HIV; Infant; Laboratories; Life; Macaca; Macaca nemestrina; Maintenance; Modeling; Primates; Quarantine; Reproducibility; Research; Research Project Grants; Resources; SIV; Serology; Simian Retroviruses; Source; Supervision; Testing; United States National Institutes of Health; Universities; Viral; Virus; germ free condition

Abstract To enhance the rigor and reproducibility of SIV/macaque models of HIV/AIDS, it is essential to provide specific pathogen free (SPF) macaques to the research community. The NIH/ORIP has supported the development and maintenance of SPF macaque colonies since 2000 as a critical biomedical research resource particularly for SIV/macaque models of HIV/AIDS. Every pigtail macaque currently in the Johns Hopkins University SPF breeding colony meets the 4 viral agent SPF criteria set by NIH/ORIP, testing negative for Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV-1). The SPF status of the JHU pigtail macaque breeding colony has been maintained for over ten years with no breaks detected by ongoing surveillance. The Virus Reference Laboratory (VRL, San Antonio, TX) performs all of our primate serology and PCR assays. Under the supervision of Dr. Eric Hutchinson, core director, all of the pigtail macaques at Johns Hopkins are tested annually by serology for the four SPF viral agents. In addition, all colony-born infants undergo two sequential PCR tests for SRV and STLV during the first year of life. Any rare additions to the colony from sources outside JHU are similarly tested by repeat PCR upon arrival in JHU quarantine. Our goal is to continue maintenance of SPF status of a valuable macaque national research resource for HIV/AIDS research. The specific aim of this Viral Testing Core is to maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV-1).

摘要 为了提高HIV/AIDS的SIV/猕猴模型的严谨性和可重复性，必须 为研究界提供无特定病原体（SPF）猕猴。NIH/ORIP 自2000年以来，支持SPF猕猴群体的发展和维护， 生物医学研究资源，特别是SIV/艾滋病毒/艾滋病猕猴模型。每一根辫子 目前在约翰霍普金斯大学SPF繁殖群的猕猴符合4种病毒因子 NIH/ORIP设定的SPF标准，猴疱疹病毒1型（B病毒）检测阴性， 猴免疫缺陷病毒（SIV）、猴逆转录病毒（SRV）和猴T淋巴细胞嗜 病毒（STLV-1）。JHU猪尾猕猴繁殖群的SPF状态已被 保持超过十年，通过持续监测未发现断裂。病毒 参考实验室（VRL，圣安东尼奥，德克萨斯州）执行我们所有的灵长类动物血清学和PCR 分析。在核心主管埃里克哈钦森博士的监督下，所有的猪尾猕猴 每年对约翰霍普金斯医院的所有患者进行四种SPF病毒因子的血清学检测。酒店客房经过精心 在出生后的第一年，克隆出生的婴儿接受了两次连续的SRV和STLV PCR检测。 生活从JHU以外来源向殖民地中添加的任何罕见添加物均通过重复进行类似检测 抵达JHU隔离区后进行PCR。我们的目标是继续保持SPF状态， 宝贵的猕猴国家研究资源的艾滋病毒/艾滋病研究。其具体目的是 病毒检测核心是通过定期血清学和PCR来维持殖民地的SPF状态 检测4种病毒因子：猕猴疱疹病毒1型（B病毒）、猿猴免疫缺陷 病毒（SIV）、猿猴逆转录病毒（SRV）和猿猴嗜T淋巴细胞病毒（STLV-1）。