Viral Testing Core

摘要 为了提高艾滋病毒/艾滋病SIV/猕猴模型的严密性和重复性，至关重要的是 向研究社区提供无特定病原体(SPF)的猕猴。NIH/ORIP已经 自2000年以来支持SPF猕猴群体的开发和维护，作为一项关键 生物医学研究资源，特别是艾滋病毒/艾滋病的SIV/猕猴模型。每条辫子 猕猴目前在约翰霍普金斯大学的SPF繁殖群体中遇到了4种病毒制剂 NIH/ORIP制定的SPF标准，脑疱疹病毒1型(B病毒)检测呈阴性， 猿猴免疫缺陷病毒(SIV)、猿猴逆转录病毒(SRV)和猿猴T淋巴细胞营养 病毒(STLV-1)。JHU小尾猴繁殖群体的SPF状态已被 保持了十多年，持续监测没有发现任何断裂。病毒 参考实验室(VRL，圣安东尼奥，德克萨斯州)执行我们所有的灵长类血清学和聚合酶链式反应 化验。在核心主任Eric Hutchinson博士的监督下，所有的辫子猕猴 在约翰霍普金斯大学，每年都要进行四种SPF病毒制剂的血清学检测。此外，所有 殖民地出生的婴儿在#年的第一年接受了两次SRV和STLV的连续PCR检测 生活。来自JHU以外来源的任何罕见的菌落添加都要进行类似的重复测试 在抵达JHU检疫时进行聚合酶链式反应。我们的目标是继续维护SPF状态 为艾滋病毒/艾滋病研究提供宝贵的猕猴国家研究资源。这样做的具体目的是 病毒检测的核心是通过定期的血清学和聚合酶链式反应来维持菌落的SPF状态 4种病原体的检测：脑炎疱疹病毒1型(B病毒)，猿猴免疫缺陷 病毒(SIV)、猿猴逆转录病毒(SRV)和猿猴T淋巴细胞营养病毒(STLV-1)。

Abstract To enhance the rigor and reproducibility of SIV/macaque models of HIV/AIDS, it is essential to provide specific pathogen free (SPF) macaques to the research community. The NIH/ORIP has supported the development and maintenance of SPF macaque colonies since 2000 as a critical biomedical research resource particularly for SIV/macaque models of HIV/AIDS. Every pigtail macaque currently in the Johns Hopkins University SPF breeding colony meets the 4 viral agent SPF criteria set by NIH/ORIP, testing negative for Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV-1). The SPF status of the JHU pigtail macaque breeding colony has been maintained for over ten years with no breaks detected by ongoing surveillance. The Virus Reference Laboratory (VRL, San Antonio, TX) performs all of our primate serology and PCR assays. Under the supervision of Dr. Eric Hutchinson, core director, all of the pigtail macaques at Johns Hopkins are tested annually by serology for the four SPF viral agents. In addition, all colony-born infants undergo two sequential PCR tests for SRV and STLV during the first year of life. Any rare additions to the colony from sources outside JHU are similarly tested by repeat PCR upon arrival in JHU quarantine. Our goal is to continue maintenance of SPF status of a valuable macaque national research resource for HIV/AIDS research. The specific aim of this Viral Testing Core is to maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV-1).