Continued support for an SPF Macaca nemestrina breeding colony to provide animals to NIH funded HIV research projects

摘要 这个项目的目的是提供健康的，行为正常的，MHC特征的，SPF猪尾巴 NIH资助的艾滋病毒/艾滋病相关研究的猕猴。我们将扩大目前SPF育种的规模 增加可供繁殖的雌性和可供出售的幼年雄性和雌性的数量 由美国国立卫生研究院资助的约翰霍普金斯大学和其他研究机构的艾滋病毒/艾滋病研究人员。 完整的谱系和健康数据被保存下来，并在动物被 被选中出售。病毒筛查包括对4种病毒剂进行定期血清学和聚合酶链式反应检测：头孢吡辛 疱疹病毒1型(B病毒)、猿猴免疫缺陷病毒(SIV)、猿猴逆转录病毒(SRV)和猿猴T- 淋巴营养病毒(STLV)。在过去的十年里，这个殖民地的检测结果一直是阴性的 对这些特工来说。在#年的第一年，对所有的青少年进行主要组织相容性(MHC)分析 并在动物被选择购买时提供给调查人员。在过去五年中， 该殖民地每年平均产生51只后代，导致平均出售32只动物。我们 据估计，每年出售42头动物将使殖民地实现自给自足，这是我们计划的目标 我们将在2028年底达到。

Abstract The aims of this project are to provide healthy, behaviorally normal, MHC-characterized, SPF pig-tailed macaques for NIH-funded HIV/AIDS related research. We will expand the size of the current SPF breeding colony to increase the numbers of females for breeding and juvenile males and females available for sale to NIH-funded HIV/AIDS investigators both within Johns Hopkins University and at other research institutions. Complete pedigrees and health data are maintained and provided to investigators when animals are being selected for sale. Viral screening includes regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV), and Simian T- lymphotrophic Virus (STLV). For the past decade the colony has consistently and repeatedly tested negative for these agents. Major histocompatibility (MHC) profiles are performed on all juveniles during the first year of life and are provided to investigators when animals are being selected for purchase. Over the last five years, the colony has produced an average of 51 offspring each year, resulting in an average of 32 animals sold. We estimate that selling 42 animals per year would allow the colony to achieve self-sufficiency, a goal we project we will reach by the end of 2028.