Analytical Chemistry Core (ACC)

摘要 杜克大学超级基金研究中心(DUSRC)将研究神经发育健康的影响 早期接触多环芳烃和金属的共同作用。分析化学核心(ACC)的总体功能是 提供与超级基金有关的污染物的常规样本分析以及暴露的生物标志物 DUSRC内的研究项目。此外，行政协调委员会还提供分析专门知识，以确定 利用高分辨质谱法研究这些超级基金污染物的转化产物和/或代谢物 光谱技术。在过去的供资期间，行政协调会一直是刚果民主共和国的一个重要组成部分， 包括开发新的有机和无机化合物分析方案以及分析 超过2,000个样品，用于各种分析物(例如，阻燃剂、有机磷农药、 生物和环境样品中的多环芳烃和痕量金属)。在应用高科技方面取得了进展。 分辨率质谱学和其他方法用于非靶向和效应导向的新药分析 超级基金相关化学品在环境和生物中的污染物和转化产物 媒体。在此，我们建议继续并扩大行政协调会的活动，重点是有针对性地分析 生物和环境样品中的多环芳烃、金属、神经递质生物标志物以及非靶标和 多环芳烃化合物的转化产物分析。此外，我们建议行政管理委员会发挥更大作用，以 通过分析复杂的环境混合物(例如，渗滤液)协调跨项目协作 房屋灰尘、多环芳烃生物降解产品)，将由项目1和5产生，并与项目共享 2、3和4，用于评价毒性潜力。行政协调委员会将参与研究经验和培训 领导与分析化学相关的培训和咨询活动。ACC团队还将提供 关于样品制备方法和仪器分析培训的非正式实践指导。我们 预期这些交互作用将有助于指导研究人员为他们的研究活动进行实验设计 这将需要进行化学分析。最后，我们将利用我们的专业知识来开发分析方法和 对试验期间可能出现的其他超级基金相关化合物进行分析 复杂的混合物和在超级基金网站上的研究。这些活动将借鉴我们迄今取得的相当大的成功 开发、验证和应用环境和生物中的污染物分析方法 样本。

Abstract The Duke University Superfund Research Center (DUSRC) will examine the neurodevelopmental health impacts of early-life co-exposures to PAHs and metals. The overall function of the Analytical Chemistry Core (ACC) is to provide routine sample analysis of Superfund-relevant contaminants as well as biomarkers of exposure for Research Projects within the DUSRC. Furthermore, the ACC provides analytical expertise in identifying transformation products and/or metabolites of these Superfund contaminants using high-resolution mass spectrometry techniques. The ACC has been an essential component of DUSRC over the past funding period, including the development of new analytical protocols for organic and inorganic compounds and the analysis of more than 2,000 samples for a wide variety of analytes (e.g., flame retardants, organophosphate pesticides, PAHs, and trace metals) in biological and environmental samples. Progress has been made in applying high- resolution mass spectrometry and other methods for non-targeted and effects-directed analysis of novel contaminants and transformation products of Superfund-relevant chemicals in environmental and biological media. Here, we propose to continue and extend the activities of the ACC, with a focus on targeted analysis of PAHs, metals, neurotransmitter biomarkers in biological and environmental samples as well as non-targeted and transformation product analysis of PAH compounds. In addition, we propose an increased role for the ACC to coordinate cross-project collaborations through the analysis of complex environmental mixtures (e.g., leachates of house dust, PAH biodegradation products) that will be generated by Projects 1 and 5, and shared to Project 2, 3, and 4 for evaluation of toxicity potential. The ACC will engage with the Research Experience and Training Core by leading training and consulting activities related to analytical chemistry. The ACC team will also offer informal hands-on instruction for training on sample preparation methods and instrumental analyses. We anticipate that these interactions will help guide researchers on experimental design for their research activities that will require chemical analyses. Finally, we will leverage our expertise to develop analytical methods and perform analyses of other Superfund-relevant compounds that may emerge during the experimentation of complex mixtures and study at Superfund sites. These activities will draw on our considerable success to date in developing, validating, and applying methods for contaminant analysis in environmental and biological samples.