Raman spectroscopic platform for transcutaneous monitoring of bone quality
Raman spectroscopic platform for transcutaneous monitoring of bone quality
批准号:
10658546
负责人:
Hani A Awad
金额:
$57.08万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
未结题
起止时间:
2016-07-01 至 2028-04-30
关键词:
AddressAffectAgeAlgorithmsAmericanAnatomyBiochemicalBody mass indexBone DensityBone DiseasesCadaverClassificationClinicClinical ResearchComplementDataData SetDiagnosisDiagnosticDigital X-RayDistalDual-Energy X-Ray AbsorptiometryEarly InterventionEarly identificationEconomic BurdenEnrollmentFemaleFoundationsFractureFundingFutureHandHeterogeneityHip FracturesHip region structureHumanHuman VolunteersIncidenceLibrariesLightingLimb structureLocationMeasurementMeasuresMedical centerMetacarpal boneMethodologyMethodsMineralsMonitorMonte Carlo MethodMusOpticsOsteoporosisPatientsPeripheralPhalanxPhotonsPhysicians&apos OfficesPrevalenceProtocols documentationRaceRadius FracturesRaman Spectrum AnalysisReportingReproducibilityRetrospective StudiesRiskRisk AssessmentRisk EstimateSafetySamplingScanningScreening procedureSensitivity and SpecificitySignal TransductionSiteSkinSourceSpectrum AnalysisStandardizationThickTissuesUnited States National Institutes of HealthVertebral columnVisitWomanWristaccess disparitiesarmbiomechanical testbonebone fragilitybone healthbone qualitybone strengthclinically relevantcommunity clinicdata acquisitiondetectordosagefallsfracture riskfragility fractureglobal healthimprovedin vivoinstrumentationirradiationmalemenmouse modelnovelosteoporotic boneprimary care providerresponsescale upscreeningsexskin colorsocioeconomicssoft tissuespine bone structuretoolvolunteerwrist fracture
中文摘要
骨质疏松症(OP)是一个全球性的健康问题,具有巨大的社会经济负担。令人震惊的是,
使用标准双能X射线吸收测定法(DXA)进行骨质疏松症筛查的结果非常糟糕,
可预防的脆性骨折的高发病率。我们的长期目标是发展一种无障碍的筛查
一种基于拉曼光谱(RS)的方法,用于在就诊期间早期准确识别高危患者,
初级保健提供者或社区诊所。我们认为,这将增加对金本位制的推荐
DXA诊断和早期干预,以减少可预防的脆性骨折的发生率。我们
先前证明RS检测与以下相关的生化变化的可靠性和灵敏度:
骨疾病的各种小鼠模型,并报告了强大的相关性,拉曼光谱特征与
全骨强度和断裂韧性。我们还开发了仪器和复杂的算法
从覆盖的软组织中减去光学贡献
完整肢体上鼠骨的经皮RS(tRS)。当我们转向扩大我们的仪器,
在人类的诊断测量中,我们正在解决三个重大挑战。第一,制造能力
可靠的经皮骨测量受到来自厚软组织层的信号的阻碍,
覆盖骨头因此,我们确定了指骨和掌骨在手中的解剖部位
适用于tRS测量,这可以通过调整照明源-检测器偏移来实现
建立各种组织的光谱库。第二,目前没有可证明的关联
手部周围骨的RS与脆性骨折的临床相关部位的BMD之间的关系,
如手腕、臀部和脊柱。第三,目前没有证据表明骨的RS可以安全可靠地
用于骨骼健康诊断。在目标1中,我们将调整我们小组的新光谱
解混算法,SOLD,将其应用于最近获得的人尸体手tRS数据集
测量.该适应将解释在200 nm处的拉曼光谱响应中的空间不均匀性。
骨干中段和骨骺区在目标2中,我们将证明tRS之间的诊断关联
尸体手指骨和掌骨的测量与临床相关的腕和髋DXA
T评分和腕关节骨折风险。尸体手将从不同性别,年龄,
种族、BMI和DXA BMD和T评分。在目标3中,我们将在50名志愿者中开展一项试验性体内tRS研究,
改进方法学,识别尸体数据差异,并直接进行骨折风险评估
tRS数据。拟议的研究代表了证明概念验证的基本步骤,
经皮拉曼光谱作为骨质疏松症的临床相关诊断和预筛选工具。
该项目的成功完成将为今后的临床研究奠定基础。
英文摘要
Osteoporosis (OP) is a global health concern with enormous socioeconomic burdens. Alarmingly, rates of
osteoporosis screening using standard dual-energy x-ray absorptiometry (DXA) are abysmal, which contributes
to high rates of preventable fragility fractures. Our long-term objective is to develop an accessible screening
method based on Raman spectroscopy (RS) for early, accurate identification of at-risk patients during visits to
primary care providers or community clinics. We posit that this would increase referrals for a gold standard
DXA diagnosis and earlier interventions to reduce the incidence of preventable fragility fractures. We
previously demonstrated the reliability and sensitivity of RS to detect biochemical changes associated with
bone diseases in various mouse models and reported robust correlations of Raman spectral features with
whole bone strength and fracture toughness. We also developed instrumentation and sophisticated algorithms
to subtract optical contributions from overlying soft tissue to enable reliable diagnostically sensitive
transcutaneous RS (tRS) of murine bone on intact limbs. As we pivot to scale up our instrumentation to make
diagnostic measurements in humans, we are addressing three significant challenges. First, the ability to make
reliable transcutaneous bone measurements is hampered by the signal from the thick layers of soft tissues that
overlay the bone. Therefore, we identified the phalanges and metacarpals in the hand as anatomical sites
suitable for tRS measurements, which can be accomplished by adjusting illumination source-detector offsets
and establishing spectral libraries of various tissues. Second, there are no currently demonstrable associations
between RS of peripheral bones of the hand and BMD at the clinically relevant sites of fragility fractures such
as the wrist, hip, and spine. Third, there is currently no evidence that RS of bone can be safely and reliably
acquired in living subjects for bone health diagnosis. In Aim 1, we will adapt our group’s novel spectral
unmixing algorithm, SOLD, to apply it to a recently acquired dataset of human cadaver hand tRS
measurements. The adaptation will account for spatial heterogeneities in Raman spectral responses at the
midshaft versus the epiphyseal regions. In Aim 2 we will demonstrate diagnostic associations between tRS
measurements in the phalanges and metacarpals of cadaver hands with clinically relevant wrist and hip DXA
T-scores and wrist fracture risk. The cadaver hands will be obtained from donors with different sexes, ages,
races, BMI, and DXA BMD and T-scores. In Aim 3, we will launch a pilot in vivo tRS study on 50 volunteers to
improve methodology, identify differences from cadaver data, and perform fracture risk estimations directly
from the tRS data. The proposed studies represent essential steps to demonstrating proof-of-concept of
transcutaneous Raman spectroscopy as a clinically relevant diagnostic and prescreening tool for osteoporosis.
Successful completion of the project will lay the foundation for future clinical studies.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1002/jbio.202000256
发表时间:
2020-11
期刊:
Journal of biophotonics
影响因子:
2.8
作者:
[Chen K, Massie C, Berger AJ]
通讯作者:
Berger AJ
Calibration Technique for Suppressing Residual Etalon Artifacts in Slit-Averaged Raman Spectroscopy.
用于抑制缝隙平均拉曼光谱法中残留的Etalon伪像的校准技术。
DOI:
10.1177/00037028211046643
发表时间:
2022-03
期刊:
Applied spectroscopy
影响因子:
3.5
作者:
[Massie C, Chen K, Berger AJ]
通讯作者:
Berger AJ
Training in Musculoskeletal Science: Comprehensive Training in Pain Studies
-
批准号:10853550
-
项目类别:
-
资助金额:$11.8万
-
财政年份:2023
-
负责人:Hani A Awad
-
依托单位:
Biomechanics, Biomaterials and Multimodal Tissue Imaging Core (BBMTI Core)
-
批准号:10232836
-
项目类别:
-
资助金额:$23.39万
-
财政年份:2022
-
负责人:Hani A Awad
-
依托单位:
Training in Musculoskeletal Science
-
批准号:10655484
-
项目类别:
-
资助金额:$31.47万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
Training in Musculoskeletal Science
-
批准号:10405447
-
项目类别:
-
资助金额:$30.51万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
A microphysiological system of tendon inflammation and fibrosis for drug screening and efficacy testing
-
批准号:10515790
-
项目类别:
-
资助金额:$74.04万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
A microphysiological system of tendon inflammation and fibrosis for drug screening and efficacy testing: MPS Database Engagement
-
批准号:10430792
-
项目类别:
-
资助金额:$7.54万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
A microphysiological system of tendon inflammation and fibrosis for drug screening and efficacy testing
-
批准号:10239102
-
项目类别:
-
资助金额:$75.53万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
A microphysiological system of tendon inflammation and fibrosis for drug screening and efficacy testing
-
批准号:10674534
-
项目类别:
-
资助金额:$72.19万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
A microphysiological system of tendon inflammation and fibrosis for drug screening and efficacy testing
-
批准号:10037991
-
项目类别:
-
资助金额:$76.55万
-
财政年份:2020
-
负责人:Hani A Awad
-
依托单位:
Project 1: Elucidating the Mechanisms of S. aureus Motility in Bone and Developing Interventions
-
批准号:10247795
-
项目类别:
-
资助金额:$15.33万
-
财政年份:2017
-
负责人:Hani A Awad
-
依托单位:
Elucidating the Mechanisms of S. aureus Motility in Bone and Developing Interventions
-
批准号:10402966
-
项目类别:
-
资助金额:$35.97万
-
财政年份:2017
-
负责人:Hani A Awad
-
依托单位:
Raman spectroscopic platform for transcutaneous monitoring of bone quality
-
批准号:9194033
-
项目类别:
-
资助金额:$43.75万
-
财政年份:2016
-
负责人:Hani A Awad
-
依托单位:
Raman spectroscopic platform for transcutaneous monitoring of bone quality
-
批准号:9274907
-
项目类别:
-
资助金额:$43.38万
-
财政年份:2016
-
负责人:Hani A Awad
-
依托单位:
Stem Cells and 3D- printed Biomaterials for Craniofacial Critical Defect Regeneration
-
批准号:9000891
-
项目类别:
-
资助金额:$21.1万
-
财政年份:2015
-
负责人:Hani A Awad
-
依托单位:
Biomechanics and Multimodal Tissue Imaging Core
-
批准号:8186757
-
项目类别:
-
资助金额:$30.59万
-
财政年份:2011
-
负责人:Hani A Awad
-
依托单位:
Noninvasive optical monitoring of bone quality in an arthritic mouse model
-
批准号:8233971
-
项目类别:
-
资助金额:$17.31万
-
财政年份:2011
-
负责人:Hani A Awad
-
依托单位:
Noninvasive optical monitoring of bone quality in an arthritic mouse model
-
批准号:8091837
-
项目类别:
-
资助金额:$16.77万
-
财政年份:2011
-
负责人:Hani A Awad
-
依托单位:
Molecular Multispectral Imaging
-
批准号:7793217
-
项目类别:
-
资助金额:$37.01万
-
财政年份:2010
-
负责人:Hani A Awad
-
依托单位:
Allografts and Gene Therapy in Flexor Tendon Tissue Engineering
-
批准号:7876730
-
项目类别:
-
资助金额:$34.08万
-
财政年份:2009
-
负责人:Hani A Awad
-
依托单位:
Allografts and Gene Therapy in Flexor Tendon Tissue Engineering
-
批准号:8478043
-
项目类别:
-
资助金额:$31.39万
-
财政年份:2009
-
负责人:Hani A Awad
-
依托单位:
海外基金