COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA

灰霉病菌分泌蛋白质的比较蛋白质组学和糖蛋白质组学

• 批准号: 7602803
• 负责人: RON ORLANDO
• 金额: $ 4.32万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602803
• 关键词: Affect; Botrytis; Cell Wall; Computer Retrieval of Information on Scientific Projects Database; Development; Economics; Enzymes; Flowers; Funding; Grant; Gray unit of radiation dose; Growth; Harvest; Infection; Institution; Molds; Numbers; Phase; Plants; Prevention strategy; Process; Proteins; Proteome; Proteomics; Recombinant Proteins; Research; Research Personnel; Resources; Source; Tissues; Trees; United States National Institutes of Health; comparative; fruits and vegetables; fungus; glycosylation; pathogen; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The fungus Botrytis cinerea is a widespread plant pathogen that affects many vegetables and fruits, as well as a large number of shrubs, trees, flowers, and weeds by causing grey mold or soft rot on the host tissues. B. cinerea mainly infects dicotyledons and non-graminaceous monocotyledons, and the infection not only occurs during the growth phase of crops but also during storage of the harvested products. Thus, it is responsible for significant economic losses. A great deal of research has focused on understanding the B. cinerea infection process, which in turn directs the development of effective prevention strategies. B. cinerea must breach the cell wall during the infection process and accordingly secretes a great number of enzymes that can degrade cell walls. Little is known concerning the identity of these enzymes. The majority of these cell wall degrading enzymes are glycosylated. Although the glycosylation on several recombinant proteins have been characterized, very little is known on the native enzymes, despite the importance of glycosylation on protein structure and activity. To this end, we have started to characterize the secreted proteome and glycoproteome of this fungus.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 真菌辣椒粉是一种广泛的植物病原体，影响了许多蔬菜和水果，也是一个大的植物病原体 灌木，树木，花和杂草的数量通过在宿主组织上引起灰色模具或软腐烂。 B.灰果会主要感染 双子叶植物和非碎量单子叶植物，不仅发生在农作物的生长阶段 而且还在储存收获产品期间。因此，它是造成重大经济损失的原因。很多 研究的重点是理解B. cinerea感染过程，这又指导了有效的发展 预防策略。 B.灰果会在感染过程中违反细胞墙，因此分泌了一个伟大的 可以降解细胞壁的酶数量。关于这些酶的身份知之甚少。大多数 这些细胞壁降解酶是糖基化的。尽管几种重组蛋白上的糖基化已经 表征，尽管糖基化对蛋白质结构和 活动。为此，我们已经开始表征这种真菌的分泌蛋白质组和糖蛋白酶。