MIRA: Systems genomics of complex traits - Administrative Supplements to Support Undergraduate Summer Research Experiences

MIRA：复杂性状的系统基因组学 - 支持本科生暑期研究经验的行政补充

• 批准号: 10810171
• 负责人: Patrick C. Phillips
• 金额: $ 0.93万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10810171
• 关键词: Administrative Supplement; Affect; Caenorhabditis elegans; Complex; Computational Biology; Disease; Engineering; Environment; Environmental Risk Factor; Evolution; Gene Expression Profile; Genes; Genetic; Genetic Recombination; Genetic Transformation; Genetic Variation; Genomics; Genotype; Health; Human; Individual; Knock-out; Maps; Microfluidics; Phenotype; Population; Predisposition; Regulatory Element; Research; Sexual Reproduction; Shapes; Source; Structure; System; Testing; Variant; experience; genetic approach; human disease; pleiotropism; prevent; response; single cell analysis; success; summer research; trait; undergraduate student

Project Summary: The genotype-phenotype map in humans is shaped by complex interactions between multiple loci and environmental factors, but the structure and function of these interactions remain poorly understood. This lack of understanding limits our ability to predict and prevent individual susceptibility to sickness and disease. These interactions can generate pleiotropic effects that confound traditional knockout approaches. Moreover, the genomic context can modulate these interactions both functionally and evolutionarily. We aim to elucidate these questions using a systems-genetics framework with three experimental paradigms in Caenorhabditis elegans and its relatives: (1) How do complex regulatory systems evolve and function? (2) What are the sources and consequences of genomic hyper diversity?, and (3) How do variation, recombination and sexual reproduction affect evolutionary responses? We will employ an integrative approach that combines genomics, single-cell analysis, microfluidic engineering, high-throughput phenotyping, genetic transformation, and computational biology. This research combines a strong functional hypothesis-testing framework with natural genetic variation to apply a systems-genetics approach that helps understand the evolution and function of complex regulatory systems that have critical implications for human health.

项目总结:

项目成果

Post-insemination selection dominates pre-insemination selection in driving rapid evolution of male competitive ability.

• DOI: 10.1371/journal.pgen.1010063
• 发表时间: 2022-03
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Kasimatis KR;Moerdyk-Schauwecker MJ;Lancaster R;Smith A;Willis JH;Phillips PC
• 通讯作者: Phillips PC

Sexual Dimorphism through the Lens of Genome Manipulation, Forward Genetics, and Spatiotemporal Sequencing.

• DOI: 10.1093/gbe/evaa243
• 发表时间: 2021-02-03
• 期刊: Genome biology and evolution
• 影响因子: 3.3
• 作者: Kasimatis KR;Sánchez-Ramírez S;Stevenson ZC
• 通讯作者: Stevenson ZC

Post-insemination sexual selection in males indirectly masculinizes the female transcriptome.

雄性的授精后性选择间接使雌性转录组男性化。

• DOI: 10.1101/2023.08.09.552689
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Kasimatis,KatjaR;Willis,JohnH;Phillips,PatrickC
• 通讯作者: Phillips,PatrickC

Opposing directions of stage-specific body shape change in a close relative of C. elegans.

• DOI: 10.1186/s40850-022-00131-y
• 发表时间: 2022-07-06
• 期刊: BMC ZOOLOGY
• 影响因子: 1.6
• 作者: Hammerschmith, Eric W.;Woodruff, Gavin C.;Moser, Kimberly A.;Johnson, Erik;Phillips, Patrick C.
• 通讯作者: Phillips, Patrick C.