ULTRA-VIOLET REFRACTOMETRY OF LIVE CELLS FOR QUANTITATIVE DNA ANALYSIS

用于 DNA 定量分析的活细胞紫外折光法

• 批准号: 8364150
• 负责人: Dax Fu
• 金额: $ 2.23万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364150
• 关键词: Biomedical Research; Cells; DNA; DNA analysis; Funding; Grant; Image; Lasers; Life; Light; Maps; Microscopy; National Center for Research Resources; Organelles; Phase; Ploidies; Principal Investigator; Proteins; Refractive Indices; Refractometry; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Variant; Viola; absorption; base; cost; ultraviolet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Interferometric phase microscopy has been widely applied to living cell studies due to its inherent ability to map refractive index variation without exogenous contrast. However, in general these variations are rather weak and does not provide a strong contrast in differentiation of various organelles, but instead only provide their overall biomolecular content. In order to increase refractive index based contrast, we moved into the ultraviolet region of the light spectrum, where protein and DNA show substantial difference in absorption. Based on Kramers-Kronig relationship, we expect that their refractive index dispersion will also be markedly different and therefore can be utilized separate quantification of cellular protein and DNA content in live cell quantitative phase imaging.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心赠款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 干涉相位显微镜由于其固有的折射率变化的映射能力，没有外源的对比度，已被广泛应用于活细胞的研究。 然而，一般来说，这些变化是相当弱的，不提供各种细胞器的分化的强烈对比，而是仅提供它们的总体生物分子含量。为了增加基于折射率的对比度，我们移动到光谱的紫外区域，其中蛋白质和DNA在吸收方面显示出实质性差异。基于Kramers-Kronig关系，我们预计它们的折射率色散也将显著不同，因此可以在活细胞定量相位成像中分别定量细胞蛋白质和DNA含量。