MEMBRANE FLUCTUATIONS OF RED BLOOD CELLS WITH DIFFRACTION PHASE MICROSCOPY

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 人红细胞（RBC）膜的膜是一种复合结构，提供了细胞形态和力学的主要控制。它由连接到2D血影蛋白网络的流体脂质双层组成，该网络决定了复合膜的弹性。这种结构的动力学性质影响RBC在循环中运输氧的能力。目前用于评估红细胞粘弹性的技术依赖于施加外部载荷，并且在获得宽频率范围内的动态信息方面受到限制。我们应用非接触式光学干涉技术来量化红细胞膜在很宽的空间和时间频率范围内的热波动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The membrane of a human red blood cell (RBC) membrane is a composite structure that provides the principal control of both the cell's morphology and mechanics. It consists of a fluid lipid bilayer tethered to a 2D spectrin network, which determines the composite membrane's elasticity. The dynamic properties of this structure influence the ability of RBCs to transport oxygen in circulation. Current techniques for assessing the viscoelasticity of RBCs rely on applying external loads and are limited in obtaining dynamic information over a wide range of frequencies. We apply a non-contact optical interferometric technique to quantify the thermal fluctuations of RBC membranes over a broad range of spatial and temporal frequencies.