SPECKLE FIELD PHASE MICROSCOPY

散斑场相位显微镜

• 批准号: 8170412
• 负责人: YOUNGKYU PARK
• 金额: $ 1.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170412
• 关键词: Biological; Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Life; Light; Lighting; Microscopy; Noise; Optics; Pattern; Phase; Research; Research Personnel; Resolution; Resources; Sampling; Scanning; Source; Staging; Techniques; Three-Dimensional Imaging; United States National Institutes of Health; cellular imaging; electric field; improved; lens

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. We developed a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrated high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在传统的全息相位显微术（HPM）中使用相干光有三个主要缺点：空间分辨率差，深度切片弱，以及由于不必要的衍射而产生的固定图案噪声。我们开发了一种可以克服这些缺点的技术，但保留了相位显微镜的优点-高对比度活细胞成像和3D成像。使用复杂空间图案的散斑光束进行照明，以减少固定图案噪声并提高光学切片能力。通过记录散斑的电场，我们展示了高对比度3D活细胞成像，而不需要轴向扫描-既不需要物镜透镜也不需要样品台。该技术在研究生物样品方面具有很大的潜力，具有更高的灵敏度、分辨率和光学切片能力。