TRANSEPITHELIAL ION TRANSPORT IN MOSQUITO HINDGUT CONTROLLED BY PEPTIDE HORMONE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们建议研究蚊子排泄上皮细胞的跨上皮离子转运机制及其由肽激素离子转运肽调控。冈比亚按蚊生活在淡水中，需要通过后肠和肛门乳头主动吸收离子。在吸血期间和吸血之后，成年蚊子在短时间内处理大量的液体食物。我们假设，离子转运肽（ITP）是一个主要的球员在控制蚊子的调节功能，因为它是已知的蝗虫。An中离子转运肽的基础生物学。将利用各种分子和生物化学技术对冈比亚进行研究。我们将特别询问ITP亚型表达和分泌的时间和地点。将通过测量水转运速率和电生理学方法研究ITP亚型在马氏管和后肠上的活性。拟议的研究将提供内分泌系统如何控制蚊子跨上皮离子运输的知识。了解基本的媒介生物学和生理学将提供合理的方法来开发环境友好的杀虫化合物，有助于控制媒介传播的疾病，改善人类健康。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to investigate mechanisms of transepithelial ion transport in mosquito excretory epithelial cells and its control by a peptide hormone ion transport peptide. African malaria mosquito Anopheles gambiae inhabits in fresh water, requiring active absorption of ions through hindgut and anal papillae. The adult mosquito processes a large amount of the liquid food in a short period of time during and after the blood feeding. We hypothesize that ion transport peptide (ITP) is a major player in the control of mosquito osmoregulatory function as it is known for the locust. Basic biology of the ion transport peptide in the An. gambiae will be studied using various molecular and biochemical techniques. We will specifically ask questions when and where the ITP isoforms are expressed and secreted. The activities of the ITP isoforms on malpighian tubules and hindgut will be studied by measuring the rate of water transport, and by electrophysiological methods. Proposed research will provide the knowledge how the endocrine system controls transepithelial ion transport in mosquitoes. Understanding basic vector biology and physiology will provide rational approaches to develop environmentally benign insecticidal compounds that help to control vector-born diseases, improving human health.