Large Scale T Cell Epitope Discovery: Global identification of epitopes derived from Zika (ZIKV) and Chikungunya (CHIKV) viruses following natural infection and vaccination

大规模 T 细胞表位发现：在自然感染和疫苗接种后对寨卡病毒 (ZIKV) 和基孔肯雅病毒 (CHIKV) 衍生的表位进行全面鉴定

• 批准号: 10020640
• 负责人: Alessandro Sette
• 金额: $ 88.83万
• 依托单位: LA JOLLA INSTITUTE FOR IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2020-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10020640
• 关键词: Address; Affect; Antigens; Arboviruses; Area; Binding; Biological Assay; CD8B1 gene; Chikungunya virus; Communities; Computer software; Data; Databases; Development; Diagnostic; Epitopes; Exposure to; Fever; General Population; Health; Human; Immune; Immunodominant Epitopes; In Vitro; Individual; Infection; Laboratories; Mediating; Pathogenesis; Research; Resources; Role; Support Contracts; T-Lymphocyte; T-Lymphocyte Epitopes; Testing; Vaccination; Vaccines; Validation; Virus; Work; Zika Virus; chikungunya; immunogenicity; multidisciplinary; tool; validation studies

This contract supports highly interactive, multi-disciplinary teams, whose research efforts are focused on large-scale discovery of T cell immune epitopes, validation of these epitopes, and defining their role in immune protection or immune-mediated pathogenesis in humans. Milestones include: 1) epitope identification; 2) in vitro epitope validation studies with primary human T cells to demonstrate the immunogenicity/antigenicity or diagnostic potential of all newly defined T cell epitopes shown to bind to classical or non-classical MHC molecules; and 3) submission of all epitope information and computer software to the Immune Epitope Database and Analysis Resource to facilitate access and use of the data and tools by the broader research community.

该合同支持高度互动的多学科团队，其研究工作重点是大规模发现T细胞免疫表位，验证这些表位，并确定它们在人类免疫保护或免疫介导的发病机制中的作用。里程碑包括：1）表位鉴定; 2）用原代人T细胞进行体外表位验证研究，以证明显示与经典或非经典MHC分子结合的所有新定义的T细胞表位的免疫原性/抗原性或诊断潜力;以及3）将所有表位信息和计算机软件提交给免疫表位数据库和分析资源，以促进更广泛的研究团体访问和使用数据和工具。