Mechanisms of differential responses to whole cell and acellular pertussis vaccination

项目总结 整体组件 疫苗是最具成本效益和非常成功的医疗干预措施之一。其中的大多数 疫苗依赖于CD4T细胞及其对B细胞的帮助。我们对体内、特异性人类CD4T的理解 细胞对病原体的反应仍然是模糊的，由于生物学的复杂性，细胞的稀有性，相对 难以接近的组织定位，以及识别特定CD4T细胞的技术挑战。因此，我们的 解决这一严重问题的方法一直是开发多种新技术来研究人类CD4T细胞 在过去的几年里。虽然CD4T细胞依赖的抗体反应一直是保护性的来源 对于大多数获得许可的疫苗(项目1)，有一个非常好的论点，即许多疾病 我们没有成功的疫苗需要适应性免疫反应，而不是抗体来保护 (项目2和项目3)。这里提出的三个项目积极寻求对机制的理解 通过新的实验方法调节人类抗病原体CD4T细胞。

PROJECT SUMMARY Overall Component Vaccines are one of the most cost effective and extraordinarily successful medical interventions. Most of those vaccines depend on CD4 T+ cells and their help to B cells. Our understanding of in vivo, specific human CD4+ T cell responses to pathogens remains hazy, due to the complexity of the biology, the rarity of the cells, relatively inaccessible tissue localization, and technical challenges of identifying specific CD4+ T cells. Therefore, our approach to this serious problem has been to develop multiple new techniques to study human CD4+ T cells over the past several years. While CD4+ T cell-dependent antibody responses have been the source of protection for most licensed vaccines (Project 1), there is a very good argument to be made that many of the diseases for which we do not have successful vaccines require adaptive immune responses beyond antibodies for protection (Projects 2 and 3). The three Projects proposed here vigorously pursue an understanding of the mechanisms regulating human anti-pathogen CD4+ T cells, linked by new experimental approaches.