NHGRI/DIR Animal Research Infrastructure

NHGRI/DIR 动物研究基础设施

• 批准号: 10027217
• 负责人: Daniel Kastner
• 金额: $ 80.52万
• 依托单位: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10027217
• 关键词: Accreditation; Amendment; Analgesics; Anesthesia and Analgesia; Anesthetics; Animal Care and Use Committees; Animal Experimentation; Animal Model; Animal Welfare; Animals; Area; Behavior; Behavioral Research; Biomedical Research; Blood; Caring; Collection; Disasters; Educational Status; Educational process of instructing; Ensure; Equipment; Euthanasia; Financial Support; Genotype; Guidelines; Housing; Hypersensitivity; Individual; Injections; Injury; Intake; International; Laboratory Animal Medicine; Laboratory Animals; Laboratory mice; Logistics; Medicine; Methods; Mission; Monitor; Mus; National Human Genome Research Institute; Operative Surgical Procedures; Personal Satisfaction; Preparation; Prevention; Principal Investigator; Procedures; Process; Program Evaluation; Protocols documentation; Readiness; Records; Regulation; Reporting; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Rodent; Safety; Schedule; Security; Surgeon; Suture Techniques; Techniques; Testing; Time; Training; Training Programs; Training Technics; Transgenic Organisms; United States National Institutes of Health; Update; animal care; comparative; design; environmental enrichment for laboratory animals; handbook; investigator training; meetings; occupational health/safety; programs; protocol development; response; restraint; skills

Under the OSD, the Office of Laboratory Animal Medicine (OLAM) is responsible for directing the program of laboratory animal care and use and supporting NHGRI investigators. OLAM provides all administrative functions and support to the NHGRI Animal Care and Use Committee (ACUC) to include scheduling and coordinating the logistics for monthly meetings, provides the timely preparation and distribution of agendas, minutes, decisions and related ACUC materials, provides Animal Study Proposal (ASP) design support, conducts intake protocol/amendment review to ensure completeness, maintains official electronic training records and files, organizes the semiannual program evaluation, tracks and facilitates correction of program deficiencies, organizes and assists with the response to any animal welfare concerns, performs Post Approval Monitoring (PAM) and serves as a liaison to Occupational Health and Safety and facility management. OLAM generates, maintains and updates the NHGRI Investigator Handbook, the NHGRI ACUC Handbook, ACUC guidelines, standard operating procedures (SOP), prepares and manages the Animal Program Disaster Preparedness plan and other resources to provide further information and guidance on the humane care and use of animals in research. Furthermore, OLAM provides NHGRI investigators with professional advice on comparative medicine, coordinates and organizes global animal imports/exports, processes animal orders, provides veterinary care and technical research support for investigators. OLAM continuously evaluates, develops and/or recommends refinements in the humane care and handling of laboratory animals to meet current best practices. A critical focus of investigator support is training on the proper care, use, humane treatment and handling of research animals. To this end, OLAM has created a comprehensive hands-on training program for investigators working on NHGRI research animal models. The training offered through OLAM ensures that all animal handling is in compliance with NHGRI, NIH, and Federal regulations and guidelines for the continued assurance of full accreditation by the AAALAC, International. In addition to the NIH required animal training and registering with the Animal Exposure Program (AEP), the NHGRI ACUC requires additional training prior to being listed on a NHGRI ASP or added to an ASP by amendment. This additional level of training ensures that each investigator completes and understands facility-specific requirements to include entry requirements, standard facility procedures, proper animal care and handling procedures, proper use of anesthetics and analgesics, environmental enrichment, allergy prevention, personal protective equipment and other safety requirements, security, and the procedures for reporting animal welfare concerns. The Animal Program Director (APD) or her designee performs this training. Investigators working with mice must also review the relevant sections of an NHGRI developed video, "Training in Basic Biomethodology for Laboratory Mice". This video covers the most common rodent procedures performed at NHGRI. This includes general husbandry, restraint and handling, identification methods, genotyping, injections, blood collection, anesthesia and analgesia, and euthanasia. One-on-one hands-on training with the APD or her designee occurs to assure that animals are treated humanely without injury to the animal or handler and that skills are appropriate for the experimental manipulation(s) being performed, including approved methods of euthanasia. Periodically, all Animal Study Proposals will undergo a PAM to include observing investigators perform experimental techniques to ensure congruency between the ASP and what is actually being done. Training is an on-going commitment and a high priority at NHGRI. Investigator training with OLAM occurs on an almost daily basis. OLAM also provides advanced technique training for both rodents and aquatic species. Advanced rodent training is required for individuals performing survival rodent surgery and includes viewing the video, "Training in Rodent Survival Surgery" developed and financially supported by the NHGRI and the OACU. This video covers special considerations in transgenic surgery and basic suturing techniques. Pre-surgical planning and training is initiated during protocol development. The Principal Investigator (PI) and APD will assess the proficiency of surgeons before they are allowed to perform survival surgery. Regardless of the level of expertise of the investigator, the APD observes all surgeries when they are first performed. NHGRI OLAM is committed to promoting the humane care and use of animals in biomedical and behavior research, teaching and testing.

根据OSD，实验动物医学办公室（OLAM）负责指导实验动物护理和使用计划，并支持NHGRI研究者。OLAM为NHGRI动物护理和使用委员会（ACUC）提供所有行政职能和支持，包括安排和协调每月会议的后勤工作，及时准备和分发议程、会议记录、决定和相关ACUC材料，提供动物研究提案（ASP）设计支持，进行摄入方案/修正案审查以确保完整性，维护官方电子培训记录和文件，组织半年一次的项目评估，跟踪并促进项目缺陷的纠正，组织并协助应对任何动物福利问题，执行批准后监测（PAM），并作为职业健康和安全以及设施管理的联络人。OLAM生成，维护和更新NHGRI研究者手册，NHGRI ACUC手册，ACUC指南，标准操作程序（SOP），准备和管理动物计划灾难准备计划和其他资源，以提供更多的信息和指导人道关怀和研究中使用动物。 此外，OLAM还为NHGRI研究人员提供比较医学方面的专业建议，协调和组织全球动物进出口，处理动物订单，为研究人员提供兽医护理和技术研究支持。OLAM不断评估，开发和/或建议改进实验室动物的人道关怀和处理，以满足当前的最佳实践。 研究者支持的一个关键重点是对研究动物的适当护理、使用、人道待遇和处理进行培训。为此，OLAM为NHGRI研究动物模型的研究人员创建了一个全面的实践培训计划。 通过OLAM提供的培训确保所有动物处理均符合NHGRI、NIH和联邦法规和指南，以继续确保获得AAALAC国际认证。除了NIH要求的动物培训和动物暴露计划（AEP）注册外，NHGRI ACUC在列入NHGRI ASP或通过修订添加到ASP之前还需要额外培训。这种额外的培训水平可确保每名研究者完成并理解机构特定要求，包括进入要求、标准机构程序、适当的动物护理和处理程序、麻醉剂和镇痛剂的正确使用、环境富集、过敏预防、个人防护设备和其他安全要求、安全性以及报告动物福利问题的程序。由动物项目负责人（APD）或其指定人员执行此培训。 与小鼠一起工作的研究人员还必须审查NHGRI开发的视频“实验室小鼠基本生物方法学培训”的相关部分。本视频涵盖了NHGRI最常见的啮齿动物程序。这包括一般饲养、约束和处理、识别方法、基因分型、注射、采血、麻醉和镇痛以及安乐死。与APD或其指定人员进行一对一动手培训，以确保动物得到人道对待，不会对动物或处理人员造成伤害，并且技能适用于正在进行的实验操作，包括批准的安乐死方法。 定期对所有动物研究提案进行PAM，包括观察研究者执行实验技术，以确保ASP与实际执行的内容一致。 培训是NHGRI的持续承诺和高度优先事项。 研究者几乎每天都接受OLAM培训。 OLAM还为啮齿动物和水生物种提供先进的技术培训。进行啮齿动物生存手术的个人需要高级啮齿动物培训，包括观看由NHGRI和OACU开发和资助的视频“啮齿动物生存手术培训”。这段视频涵盖了转基因手术和基本的移植技术的特殊考虑。 在方案制定期间启动术前计划和培训。 主要研究者（PI）和APD将在允许外科医生进行生存手术之前评估其熟练程度。无论研究者的专业水平如何，APD都会在首次进行手术时观察所有手术。 NHGRI OLAM致力于在生物医学和行为研究，教学和测试中促进动物的人道关怀和使用。