HERCULES: Health and Exposome Research Center

项目摘要 本项目的目的是描述二手电子烟气溶胶暴露和相关的 与每日使用电子烟的儿童一起居住的肺功能标志物。电子烟通常会蒸发液体 含有尼古丁，丙二醇（PG），植物甘油（VG），以及吸入和呼出的各种调味品 由用户有害化学品，如芳香族挥发性有机化合物（VOC），微量金属， 据报道，主流和二手电子烟气溶胶中也含有烟草特有的亚硝胺。 所有这些化学品都已知对健康有不利影响，包括对呼吸系统的影响。与 电子烟的使用迅速增加，特别是在育龄人群中，二手接触电子烟， 香烟气雾剂是一个新兴的公共卫生问题。二手电子烟烟雾对健康的影响 对旁观者，特别是对儿童的暴露尚不清楚。 我们建议采用病例对照设计，并将招募60名6-12岁的儿童（30 病例组30例，对照组30例。我们的假设是，尼古丁和芳香族挥发性有机化合物与二手电子烟有关， 气溶胶暴露将与代谢谱和代谢途径的改变有关。此外，本发明还 暴露于这些化学物质和代谢特征改变将与肺损伤的证据相关 功能使用硅胶腕带被动空气采样装置和唾液代谢组学分析，我们将： 1.检查二手电子烟气溶胶化学品暴露（尼古丁，苯， 和甲苯）和唾液代谢谱和途径。 2.检查二手电子烟化学品暴露和唾液代谢特征与吸烟的关联 肺功能的标志物（呼出一氧化氮分数、一秒用力呼气量、用力呼气量） 肺活量、呼气中期用力呼气流速和父母报告的反复/慢性呼吸道疾病 症状）。 拟议的项目将补充HERCULES Exposome中心（P30 ES 019776），并与 该中心的总体使命是利用 基于代谢组学的概念和方法，包括代谢组学。据我们所知，这将是第一个 研究二手电子烟气溶胶暴露对儿童肺功能的影响。

Project Summary This purpose of this project is to describe secondhand electronic cigarette aerosol exposure and associated markers of lung function in children who reside with daily vapers. E-cigarettes vaporize liquids typically containing nicotine, propylene glycol (PG), vegetable glycerin (VG), and various flavorings inhaled and exhaled by the user. Harmful chemicals such as aromatic volatile organic compounds (VOCs), trace metals, and tobacco-specific nitrosamines have also been reported in mainstream and secondhand e-cigarette aerosols. All of these chemicals have known adverse health effects, including effects on the respiratory system. With the rapid increase in e-cigarette use, particularly among those of child-bearing age, secondhand exposure to e- cigarette aerosol is an emerging public health concern. The health effects of secondhand e-cigarette aerosol exposure on the bystander, particularly for children, is not clear. We propose to utilize a case-control design and will recruit 60 children between the ages of 6-12 years (30 cases/30 controls). Our hypothesis is that nicotine and aromatic VOCs associated with secondhand e-cigarette aerosol exposure will associate with alterations in metabolic profiles and metabolic pathways. Further, exposure to these chemicals and metabolic profile alterations will associate with evidence of impaired lung function. Using silicone wristband passive air sampling devices and salivary metabolomics analysis, we will: 1. Examine associations between secondhand e-cigarette aerosol chemical exposures (nicotine, benzene, and toluene) and salivary metabolic profiles and pathways. 2. Examine associations of secondhand e-cigarette chemical exposure and salivary metabolic profiles with markers of lung function (fractional exhaled nitric oxide, forced expiratory volume in one second, forced vital capacity, mid-expiration forced expiratory flow rate, and parent-report of recurrent/chronic respiratory symptoms). The proposed project will supplement the HERCULES Exposome Center (P30ES019776) and aligns well with the overall Center's mission to provide a more comprehensive assessment of environmental influences using exposome-based concepts and approaches including metabolomics. To our knowledge, this would be the first study to examine the impact of secondhand e-cigarette aerosol exposure on pediatric lung function.