Olympus FV1000MPE Multiphoton Exclusive with Vision II and Chameleon OPO

描述（由申请人提供）：北卡罗来纳大学教堂山医学院神经科学中心申请资金，用于购买奥林巴斯Fluoview FV1000-MPE多光子成像系统，用于发育和再生神经系统的体内功能成像。实时和原位观察神经元增殖、迁移和分化动态的能力彻底改变了我们研究自闭症或精神分裂症等神经发育障碍的特定基因功能的能力。所要求的奥林巴斯Fluoview FV1000-MPE系统代表了一个新的“艺术状态”，因为它允许比以前更深入活组织数百微米的杰出荧光成像，提供所有商用多光子激光扫描显微镜的最高穿透深度。该系统将成为已建立的核心系统的一部分。该中心致力于为北卡罗来纳大学神经科学社区提供先进神经系统成像的有效访问和培训，成立于6年前，得到了北卡罗来纳大学教堂山医学院的大力支持，北卡罗来纳大学神经发育障碍研究中心的资金以及国家神经疾病和中风研究所的资金。尽管该设施已经支持了北卡罗来纳大学40多名神经科学研究人员的成像需求，但由于缺乏用于体内成像的专用多光子成像站，限制了有关哺乳动物大脑发育或体内损伤神经元再生的可视化项目的进展。例如，在发育完整的啮齿动物的大脑中，皮层神经元层的形成和突触连接的发育现在是可行的，但需要有能力在常规共聚焦显微镜可以看到的深度之外看到荧光标记的结构。所要求的设备将使北卡罗来纳大学神经科学中心的一批国际公认的年轻研究人员能够建立和传播与神经发育障碍研究相关的创新成像技术，并扩大最近在顶级生物医学研究期刊上发表的研究项目。我们所要求的设备的主要目标是为使用小鼠遗传模型进行体内功能研究提供一个平台，并利用它来创新和实施新的成像技术，以探测发育过程中的神经功能。

DESCRIPTION (provided by applicant): The Neuroscience Center at the UNC-Chapel Hill School of Medicine requests funding for the purchase of an Olympus Fluoview FV1000-MPE multiphoton imaging system dedicated to in vivo functional imaging of the developing and regenerating nervous system. The ability to observe the dynamics of neuronal proliferation, migration and differentiation in real time and in situ revolutionizes our ability to study the function of specific genes underlying neurodevelopmental disorders such as autism or schizophrenia. The requested Olympus Fluoview FV1000-MPE system represents a new "state of the art" as it allows outstanding fluorescence imaging hundreds of microns deeper into living tissues than previously possible, providing the highest penetration depths of all commercially available multiphoton laser scanning microscopes. This system will be part of an established Core. This Core is dedicated to provide effective access and training to the UNC Neuroscience community in advanced nervous system imaging, was established 6 years ago with strong institutional support from the UNC-Chapel Hill School of Medicine, funds from the Neurodevelopmental Disorders Research Center at UNC and funds from the National Institutes of Neurological Disorders and Stroke. Although this facility has supported the imaging needs of more than 40 neuroscience researchers at UNC, the lack of a dedicated multiphoton imaging station for in vivo imaging has restricted the progress of projects related to visualizing the development of the mammalian brain or regrowth of injured neurons in vivo. For example, visualization of cortical neuronal layer formation and development of synaptic connections in the brains of intact developing rodents is now feasible but requires the ability to visualize fluorescently labeled structures beyond depths which can be visualized by conventional confocal microscopes. The requested equipment will enable a cadre of internationally recognized young researchers at the UNC Neuroscience Center to establish and disseminate innovative imaging technologies relevant to the study of neurodevelopmental disorders and to expand research programs that have recently resulted in publications in the top biomedical research journals. Our main goals with the requested equipment are to provide a platform for in vivo functional studies using mouse genetic models and use it to innovate and implement new imaging technologies to probe neural function during development.