Olympus FV1000MPE Multiphoton Exclusive with Vision II and Chameleon OPO

描述(由申请人提供)：北卡罗来纳大学教堂山医学院的神经科学中心申请资金购买奥林巴斯FluoView FV1000-MPE多光子成像系统，致力于对正在发育和再生的神经系统进行体内功能成像。能够实时和原位地观察神经元增殖、迁移和分化的动态，这一能力彻底改变了我们研究自闭症或精神分裂症等神经发育障碍相关特定基因功能的能力。所要求的奥林巴斯FluoView FV1000-MPE系统代表了一种新的“最先进技术”，因为它允许出色的荧光成像，比以前更深入活组织数百微米，提供所有商业可用多光子激光扫描显微镜中最高的穿透深度。该系统将成为已建立的核心系统的一部分。该中心致力于为北卡罗来纳大学神经科学界提供先进神经系统成像方面的有效途径和培训，成立于6年前，得到了北卡罗来纳大学教堂山医学院的大力机构支持，北卡罗来纳大学神经发育障碍研究中心的资金，以及国家神经疾病和中风研究所的资金。尽管该设施支持了北卡罗来纳大学40多名神经科学研究人员的成像需求，但缺乏用于活体成像的专用多光子成像站限制了与可视化哺乳动物大脑发育或体内受损神经元再生相关的项目的进展。例如，对发育完整的啮齿动物大脑中皮质神经元层的形成和突触连接的发展进行可视化现在是可行的，但需要能够可视化深度以外的荧光标记结构，这是传统的共聚焦显微镜可以可视化的。所要求的设备将使北卡罗来纳大学神经科学中心的一批国际公认的年轻研究人员能够建立和传播与神经发育障碍研究相关的创新成像技术，并扩大最近在顶级生物医学研究期刊上发表的研究计划。我们所要求的设备的主要目标是提供一个使用小鼠遗传模型进行体内功能研究的平台，并利用它来创新和实施新的成像技术，以探测发育过程中的神经功能。

DESCRIPTION (provided by applicant): The Neuroscience Center at the UNC-Chapel Hill School of Medicine requests funding for the purchase of an Olympus Fluoview FV1000-MPE multiphoton imaging system dedicated to in vivo functional imaging of the developing and regenerating nervous system. The ability to observe the dynamics of neuronal proliferation, migration and differentiation in real time and in situ revolutionizes our ability to study the function of specific genes underlying neurodevelopmental disorders such as autism or schizophrenia. The requested Olympus Fluoview FV1000-MPE system represents a new "state of the art" as it allows outstanding fluorescence imaging hundreds of microns deeper into living tissues than previously possible, providing the highest penetration depths of all commercially available multiphoton laser scanning microscopes. This system will be part of an established Core. This Core is dedicated to provide effective access and training to the UNC Neuroscience community in advanced nervous system imaging, was established 6 years ago with strong institutional support from the UNC-Chapel Hill School of Medicine, funds from the Neurodevelopmental Disorders Research Center at UNC and funds from the National Institutes of Neurological Disorders and Stroke. Although this facility has supported the imaging needs of more than 40 neuroscience researchers at UNC, the lack of a dedicated multiphoton imaging station for in vivo imaging has restricted the progress of projects related to visualizing the development of the mammalian brain or regrowth of injured neurons in vivo. For example, visualization of cortical neuronal layer formation and development of synaptic connections in the brains of intact developing rodents is now feasible but requires the ability to visualize fluorescently labeled structures beyond depths which can be visualized by conventional confocal microscopes. The requested equipment will enable a cadre of internationally recognized young researchers at the UNC Neuroscience Center to establish and disseminate innovative imaging technologies relevant to the study of neurodevelopmental disorders and to expand research programs that have recently resulted in publications in the top biomedical research journals. Our main goals with the requested equipment are to provide a platform for in vivo functional studies using mouse genetic models and use it to innovate and implement new imaging technologies to probe neural function during development.