Olympus FV1000MPE Multiphoton Exclusive with Vision II and Chameleon OPO

描述（由申请人提供）：北卡罗来纳大学教堂山医学院神经科学中心申请资助购买Olympus Fluoview FV 1000-MPE多光子成像系统，该系统专用于发育和再生神经系统的体内功能成像。在真实的时间和原位观察神经元增殖、迁移和分化的动力学的能力彻底改变了我们研究神经发育障碍（如自闭症或精神分裂症）的特定基因的功能的能力。所要求的Olympus Fluoview FV 1000-MPE系统代表了一种新的“最先进技术”，因为它可以实现比以前更深入活组织数百微米的出色荧光成像，提供所有市售多光子激光扫描显微镜的最高穿透深度。该系统将成为既定核心的一部分。该核心致力于为高级神经系统成像领域的神经科学社区提供有效的访问和培训，该核心于6年前在北卡罗来纳大学教堂山医学院的强大机构支持下成立，来自神经发育障碍研究中心的资金以及来自美国国立神经疾病和中风研究所的资金。虽然该设施已经支持了40多名神经科学研究人员的成像需求，但缺乏专用的多光子成像站进行体内成像，限制了与可视化哺乳动物大脑发育或损伤神经元体内再生相关的项目的进展。例如，在完整发育的啮齿动物的大脑中，皮质神经元层形成和突触连接的发育的可视化现在是可行的，但需要能够可视化荧光标记的结构超出深度，可以通过传统的共聚焦显微镜可视化。所要求的设备将使国际公认的年轻研究人员在神经科学中心的骨干建立和传播创新的成像技术相关的神经发育障碍的研究，并扩大研究计划，最近导致在顶级生物医学研究期刊上发表。我们所要求的设备的主要目标是提供一个使用小鼠遗传模型进行体内功能研究的平台，并利用它来创新和实施新的成像技术，以探测发育过程中的神经功能。

DESCRIPTION (provided by applicant): The Neuroscience Center at the UNC-Chapel Hill School of Medicine requests funding for the purchase of an Olympus Fluoview FV1000-MPE multiphoton imaging system dedicated to in vivo functional imaging of the developing and regenerating nervous system. The ability to observe the dynamics of neuronal proliferation, migration and differentiation in real time and in situ revolutionizes our ability to study the function of specific genes underlying neurodevelopmental disorders such as autism or schizophrenia. The requested Olympus Fluoview FV1000-MPE system represents a new "state of the art" as it allows outstanding fluorescence imaging hundreds of microns deeper into living tissues than previously possible, providing the highest penetration depths of all commercially available multiphoton laser scanning microscopes. This system will be part of an established Core. This Core is dedicated to provide effective access and training to the UNC Neuroscience community in advanced nervous system imaging, was established 6 years ago with strong institutional support from the UNC-Chapel Hill School of Medicine, funds from the Neurodevelopmental Disorders Research Center at UNC and funds from the National Institutes of Neurological Disorders and Stroke. Although this facility has supported the imaging needs of more than 40 neuroscience researchers at UNC, the lack of a dedicated multiphoton imaging station for in vivo imaging has restricted the progress of projects related to visualizing the development of the mammalian brain or regrowth of injured neurons in vivo. For example, visualization of cortical neuronal layer formation and development of synaptic connections in the brains of intact developing rodents is now feasible but requires the ability to visualize fluorescently labeled structures beyond depths which can be visualized by conventional confocal microscopes. The requested equipment will enable a cadre of internationally recognized young researchers at the UNC Neuroscience Center to establish and disseminate innovative imaging technologies relevant to the study of neurodevelopmental disorders and to expand research programs that have recently resulted in publications in the top biomedical research journals. Our main goals with the requested equipment are to provide a platform for in vivo functional studies using mouse genetic models and use it to innovate and implement new imaging technologies to probe neural function during development.