RESTORATION OF CELL SIGNALING AND MUSCLE PROTEIN SYNTHESIS IN THE ELDERLY

老年人细胞信号传导和肌肉蛋白合成的恢复

• 批准号: 7719198
• 负责人: Douglas Paddon-Jones
• 金额: $ 3.44万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7719198
• 关键词: Amino Acids; Blood; Computer Retrieval of Information on Scientific Projects Database; Daily; Eating; Elderly; Experimental Designs; Funding; Grant; Growth; Home environment; Individual; Injury; Institution; Intervention; Invasive; Juice; Leucine; Measures; Methodology; Monitor; Muscle; Muscle Proteins; Numbers; Patients; Population; Procedures; Process; Protein Biosynthesis; Research; Research Personnel; Resources; Risk; Role; Sampling; Signal Transduction; Source; Supplementation; Techniques; Testing; Time; Treatment Protocols; United States National Institutes of Health; Water; Week; cohort; day; diaries; direct application; experience; human FRAP1 protein; novel; nutrition; restoration; stable isotope; volunteer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Older individuals are at greater risk of experiencing a decrease in muscle mass and strength. This may be due to a number of factor, however we are focusing on the potential preventative role of nutrition - specifically a supplement consisting of the amino acid leucine. Hypothesis: We hypothesis that two weeks of leucine supplementation will restore the potential for muscle protein synthesis following a meal that has been shown to be less effective in elderly individuals compared to the young. Specific Aims and Procedures (summary): We will employ techniques to measure muscle protein synthesis (stable isotope methodology) and the mechanisms regulating the synthetic process (mTOR cell signaling) to determine if 2 weeks of leucine supplementation has a beneficial effect the potential for muscle growth in elderly. Experimental Design (summary): We will study a cohort of elderly volunteers over a 14 day period. On days 1 and 14, we will obtain blood and muscle samples to perform our tests. On days 2-13, we will provide volunteers with take-home packets of leucine (4 g each) that they will dissolve in water or juice and consume 3 times/day with their daily meals. We will provide volunteers with a diary to record their normal food intake and monitor their supplement use. Significance (summary): This supplement represents a novel, minimally invasive, preventative intervention that minimizes the additional caloric/volume load associated with previous supplement regimens and has direct application for patient populations and elderly individuals who have a much greater risk of accelerated muscle loss due to illness, injury.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景：老年人肌肉质量和力量下降的风险更大。这可能是由于许多因素，但我们专注于营养的潜在预防作用-特别是由氨基酸亮氨酸组成的补充剂。 假设：我们假设，两周的亮氨酸补充剂将恢复肌肉蛋白质合成的潜力后，已被证明是在老年人相比，年轻人有效。 具体目的和程序（总结）：我们将采用技术来测量肌肉蛋白质合成（稳定同位素方法）和调节合成过程的机制（mTOR细胞信号传导），以确定2周的亮氨酸补充是否对老年人肌肉生长的潜力具有有益影响。 实验设计（总结）：我们将在14天内研究一组老年志愿者。在第1天和第14天，我们将采集血液和肌肉样本进行检测。在第2-13天，我们将为志愿者提供带回家的亮氨酸包（每个4 g），他们将溶解在水或果汁中，每天3次随餐服用。我们将为志愿者提供日记，记录他们的正常食物摄入量并监测他们的补充剂使用情况。 意义（总结）：这种补充剂代表了一种新型的、微创的预防性干预，其最大限度地减少了与先前补充剂方案相关的额外热量/体积负荷，并直接应用于由于疾病、损伤而具有更大的加速肌肉损失风险的患者群体和老年人。