Mitochondrial R-loop in sepsis-induced cardiomyopathy
脓毒症诱发的心肌病中的线粒体 R 环
基本信息
- 批准号:10586871
- 负责人:
- 金额:$ 44.52万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-01-12 至 2027-12-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAdaptive Immune SystemAlternative TherapiesArchitectureAutomobile DrivingBindingCardiac MyocytesCardiomyopathiesCardiovascular systemCellsChromatinChronicClinicalClinical ResearchDNADNA MethylationDataDefectDisease ProgressionEngineeringEnhancersEpigenetic ProcessFailureFunctional disorderFutureGeneticGenetic TranscriptionGenome MappingsGenomic InstabilityGoalsHeartHeart DiseasesHeart failureHybridsImmuneImmune System DiseasesImmune responseImmunityImmunotherapyInfectionInfiltrationInflammationInflammatoryInflammatory ResponseInterventionKnowledgeLifeLinkMacrophageMediatingMedicineMitochondriaMitochondrial DNAMitochondrial MatrixMolecularMonitorMorbidity - disease rateMusMyocardial dysfunctionNamesNucleic AcidsOrganOutcomePathologicPathway interactionsPatientsPatternPilot ProjectsPlayProcessRNARecoveryRegulationReporterRoleSepsisSignal TransductionSignaling MoleculeStressStructureSumoylation PathwayTestingTherapeuticTherapeutic InterventionTimeTranscriptional ActivationTranscriptional RegulationUntranslated RNAcardioprotectioncardiovascular risk factordesignepigenomeepigenome editingepigenomicsexperimental studyextracellularheart damageheart functionimprovedintercellular communicationmitochondrial genomemortalitynovelnovel therapeutic interventionpreclinical studypreventpromoterresponsesepticseptic patientstherapeutic targettooltransmission processuptake
项目摘要
Abstract
Sepsis is a life-threatening organ dysfunction that induces a multitude of defects in immunity for sustained
periods of time after clinical recovery that is closely correlated with long-term mortality associated with septic
cardiomyopathy. Although immune-based therapies have been designed to suppress inflammatory responses,
this strategy alone has marginal benefits for sepsis patients, as the dominant molecular drivers of septic
cardiomyopathy-associated inflammation remain unclear. As our knowledge of mitochondria advances, we first
investigated a previously underappreciated activator in inflammation and disease progression, mitochondrial R-
loop (RNA:DNA hybrid) termed mtR-loop, and discovered aberrant accumulation in cardiomyocytes after sepsis.
The contributory role of mtR-loop toward cardiomyopathy post sepsis was documented in a pilot study showing
that unresolved mtR-loop is pathological, which is linked to not only mitochondrial genome instability, but also
unresolved chronic inflammation through activating both non-immune cells (cardiomyocytes) and immune cells
(macrophages). Most importantly, these cardiomyocyte-derived mtR-loop could translocate to extracellular
compartments and were sensed by macrophages in the heart to fuel a fata response to sepsis. This proposal
will test the hypothesis that septic cardiomyocyte derived mtR-loop are the central hub that connect various
pathways of the immune response in the inflammatory network. If identified, mtR-loop inhibition will be explored
as a potential novel cardio-protector against stress. To this, we will employ genetic tools combined with reporter
mice to monitor mtR-loop translocation within cells, track mtR-loop uptake by immune cells, define the
mechanism underlying mtR-loop intercellular communication and initiated inflammation, and characterize the
infiltrating cells. Mechanistically, our pilot data first showed that the SUMOlyation-dependent packaging of
lncRNA (lncRP11) into mitochondria, which hybrids with mtDNA to form mtR-loop (lncRNA:mtDNA structure).
Those lncRNA-associated mtR-loop acts an upstream trigger of inflammatory network in septic hearts. Based
on these initial findings, we hypothesize that suppression of lncRP11 transcription can reduce or prevent cardiac
dysfunction in post-sepsis by directly reversing or ameliorating inflammation associated with cardiomyocyte
failure. Specifically, by 3D genome mapping, the physical contact of enhancer and promoter, but not their
activities, was identified to regulate lncRP11 transcription in septic hearts. Interestingly, this is largely due to loss
of CTCF-mediated insulator between lncRP11 promoter and enhancer. In addition, the CTCF binding on the
insulator is sensitive to DNA methylation. Thus, we propose that gain of CTCF-mediated insulator by epigenetic
editing tool could block enhancer/promoter interaction and thus silence lncRP11 transcription. Results of these
studies will be used to develop a viable therapeutic approach using targeted epigenetic editing, which can
efficiently resolve lncRP11-associated pathological mtR-loop and thereby ameliorate stress-induced cardiac
damage and reduce the mortality and morbidity of patients with septic cardiomyopathy.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Wei Huang其他文献
A new colorimetric and fluorescent ratiometric sensors for Hg2+ based on 4-pyren-1-yl-pyrimidine
基于 4-芘-1-基-嘧啶的新型 Hg2 比色和荧光比率传感器
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
Qunbo Mei;Jiena Weng;Qidan Ling;Quli Fan;Wei Huang - 通讯作者:
Wei Huang
Wei Huang的其他文献
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{{ truncateString('Wei Huang', 18)}}的其他基金
Core 1: Integrated Pathology and Radiology Core (iPRC)
核心 1:综合病理学和放射学核心 (iPRC)
- 批准号:
10555403 - 财政年份:2023
- 资助金额:
$ 44.52万 - 项目类别:
Causal role of higher-order thalamo-cortical oscillations in sustained attention
高阶丘脑皮质振荡在持续注意力中的因果作用
- 批准号:
10199754 - 财政年份:2019
- 资助金额:
$ 44.52万 - 项目类别:
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