Mosquito hydration status as a mechanism that alters pre-feeding host interactions and post-feeding physiology

蚊子的水合状态是一种改变摄食前宿主相互作用和摄食后生理机能的机制

• 批准号: 10624798
• 负责人: Josh B. Benoit
• 金额: $ 49.71万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-12 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10624798
• 关键词: Aedes; Affect; Area; Behavior; Behavior Therapy; Behavioral; Biochemical; Biological; Biology; Blood; CRISPR/Cas technology; Comparative Study; Culex pipiens; Culicidae; Dehydration; Desiccation; Detection; Development; Disease Vectors; Disease model; Droughts; Dryness; Experimental Designs; Exposure to; Genes; Goals; Habitats; High temperature of physical object; Homeostasis; Hour; Humidity; Hydration status; Immune; Immune response; Immunity; Individual; Ingestion; Insecta; Metabolism; Modeling; Mosquito-borne infectious disease; Organism; Phenotype; Physiological; Physiology; Predisposition; Proteins; RNA Interference; Reproduction; Research; Role; Seasons; Sensory; Sensory Receptors; Sindbis Virus; Stress; Temperature; Transgenic Organisms; Viral; Virus; Water; West Nile virus; Yellow Fever; Zika Virus; carbohydrate metabolism; climate variability; differential expression; disease transmission; drinking water; experimental study; feeding; genome editing; improved; knock-down; mathematical model; pathogen; preference; response; sensory mechanism; transmission process; vector; vector competence; vector mosquito; vector-borne; viral transmission

Project summary Insects are extremely prone to dehydration, where individuals may succumb after exposure to only a few hours of dry conditions. Mosquitoes are highly susceptible to desiccation due to high water loss rates, especially when temperatures are high, relative humidity is low, and drinking water is lacking. Importantly, if dehydrated mosquitoes move to more humid areas, dehydration-induced phenotypes can last for many hours. Recent studies have examined mosquito development and other specific topic areas under dry season conditions, but no integrative studies have examined the role of dehydration bouts on mosquito behavior, physiology, and potential for disease transmission. For most insects, exposure to xeric periods prompts their retreat into favorable microhabitats until conditions improve, which could take hours or weeks. Our preliminary studies indicate that activity and blood feeding in mosquitoes increase by three- to four-fold following sub-lethal dehydration, but the potential mechanisms and impact of this phenotype are unknown. The focus of this proposal will be examining the effect that dehydration has on mosquito biology, specifically how desiccation stress alters general mosquito biology, host choice, host-pathogen interactions, and disease transmission. The primary study organism will be the northern house mosquito, Culex pipiens, a vector for West Nile virus, with comparative studies to mosquito species to Aedes aegypti. These studies are supported by 1) experimental designs that can discern the effects between only exposure to dry conditions and direct mosquito dehydration (= organismal water loss), 2) preliminary studies on CRISPR-Cas9 lines of chemosensory proteins which show altered water attraction, 3) shifts in blood feeding and diversion of blood to the crop, 4) increased retention of the bloodmeal in dehydrated mosquitoes when compared to hydrated counterparts, 5) differential expression of immune genes following a bloodmeal if mosquitoes are dehydrated before host feeding, and 6) field-based mesocosm experiments that indicate dehydrated mosquitoes are more prone to blood feeding. This study has four specific aims: Specific Aim 1. Influence of dehydration on basic biological parameters before and after blood feeding. Specific Aim 2. Examine aspects underlying behavioral modifications of mosquitoes following dehydration stress. Specific Aim 3. Impact of dehydration on viral retention and transmission. Specific Aim 4. Field-based observations and mathematical modeling of disease transmission changes due to dehydration exposure. Overall goal accomplished by the completion of these proposed studies: These proposed studies will be transformative by providing the first integrative experiments that examine the effects of dehydration bouts on the dynamics between mosquito physiology, behavior, and pathogen transmission.

项目总结

项目成果

Dehydration Dynamics in Terrestrial Arthropods: From Water Sensing to Trophic Interactions.

• DOI: 10.1146/annurev-ento-120120-091609
• 发表时间: 2023-01-23
• 期刊: Annual review of entomology
• 影响因子: 23.8

The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.

• DOI: 10.1186/s12915-021-00975-9
• 发表时间: 2021-03-10
• 期刊: BMC biology
• 影响因子: 5.4
• 作者: Olafson PU;Aksoy S;Attardo GM;Buckmeier G;Chen X;Coates CJ;Davis M;Dykema J;Emrich SJ;Friedrich M;Holmes CJ;Ioannidis P;Jansen EN;Jennings EC;Lawson D;Martinson EO;Maslen GL;Meisel RP;Murphy TD;Nayduch D;Nelson DR;Oyen KJ;Raszick TJ;Ribeiro JMC;Robertson HM;Rosendale AJ;Sackton TB;Saelao P;Swiger SL;Sze SH;Tarone AM;Taylor DB;Warren WC;Waterhouse RM;Weirauch MT;Werren JH;Wilson RK;Zdobnov EM;Benoit JB
• 通讯作者: Benoit JB

Do Mosquitoes Sleep?

• DOI: 10.1016/j.pt.2020.08.004
• 发表时间: 2020-11
• 期刊: Trends in parasitology
• 影响因子: 9.6
• 作者: Ajayi OM;Eilerts DF;Bailey ST;Vinauger C;Benoit JB
• 通讯作者: Benoit JB

Tonic Immobility Is Influenced by Starvation, Life Stage, and Body Mass in Ixodid Ticks.

硬蜱的强直性不动受饥饿、生命阶段和体重的影响。

• DOI: 10.1093/jme/tjab003
• 发表时间: 2021
• 期刊: Journal of medical entomology
• 影响因子: 2.1
• 作者: Oyen,KennanJ;Croucher,Lillian;Benoit,JoshuaB
• 通讯作者: Benoit,JoshuaB

Live-bearing cockroach genome reveals convergent evolutionary mechanisms linked to viviparity in insects and beyond.

• DOI: 10.1016/j.isci.2023.107832
• 发表时间: 2023-10-20
• 期刊: ISCIENCE
• 影响因子: 5.8
• 作者: Fouks, Bertrand;Harrison, Mark C.;Mikhailova, Alina A.;Marchal, Elisabeth;English, Sinead;Carruthers, Madeleine;Jennings, Emily C.;Chiamaka, Ezemuoka L.;Frigard, Ronja A.;Pippel, Martin;Attardo, Geoffrey M.;Benoit, Joshua B.;Bornberg-Bauer, Erich;Tobe, Stephen S.
• 通讯作者: Tobe, Stephen S.