tRNA modifications as critical components of insect blood feeding and reproduction

tRNA 修饰作为昆虫血液喂养和繁殖的关键组成部分

• 批准号: 10648600
• 负责人: Josh B. Benoit
• 金额: $ 20.25万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10648600
• 关键词: Area; Arthropods; Bacteria; Biochemical; Biological; Biology; Blood; Chemicals; Codon Nucleotides; Culicidae; Data; Data Set; Enzymes; Eukaryota; Event; Fat Body; Female; Future; Gene Expression Profile; Gene Expression Profiling; Generations; Genes; Genetic Transcription; Goals; Hour; Human; Insect Vectors; Insecta; Insecticide Resistance; Life; Life Cycle Stages; Link; Methods; Midgut; Modification; Nucleosides; Nutrient; Organ; Organism; Osmosis; Output; Oxidative Stress; Partner in relationship; Pattern; Phenotype; Physiological; Physiology; Play; Population; Process; Production; Protein Biosynthesis; Publishing; RNA; Reproduction; Research; Resources; Ribonucleosides; Role; Sampling; Sex Differences; Structure; System; Testing; Time; Tissue-Specific Gene Expression; Tissues; Transcript; Transfer RNA; Untranslated RNA; Whole Organism; Yeasts; climate change; disease transmission; egg; experimental study; feeding; male; novel; pathogen; prevent; reproductive; reproductive organ; response; sex; stress tolerance; transmission process; vector; vector control

Project summary Transfer RNAs (tRNA) represent one of the most abundant forms of RNA and are required for protein synthesis. Chemically modified ribonucleosides in tRNAs play critical roles in aspects that range from decoding codons to stress tolerance. Limited studies for metazoans, specifically in blood feeding arthropods, have been conducted on tRNAs and their modifications. The goal of these studies is to provide a characterization of the tRNA pool and modifications in blood feeding insects. These changes will be examined during blood feeding, reproduction, and mating at the whole organism and tissue levels. Our studies are supported by the following: 1) preliminary results from limited samples, which identified and quantified tRNA modifications, that suggest there are differences between blood feeding and sexes; and 2) Re-analysis of previously published data sets indicate expression patterns of genes associated with tRNA modification vary following a blood meal and between sexes. This study has two specific aims: Specific Aim 1. Characterize transfer RNA modifications in insects between sexes and during blood feeding. Specific Aim 2. Investigate the role of transfer RNA modifications during blood feeding and reproduction. Upon completion of this proposed research, we will establish tRNA expression and modification profiles for multiple blood feeding species in relation to reproduction and blood feeding. The role of several modifications will be elucidated by the use of targeted reduction of specific tRNA modifying enzymes. These proposed studies will be the first to extensively examine tRNA expression and modification patterns in blood feeding insects. Our results will provide the groundwork for future, novel tRNA-based studies of blood feeding insects such as identifying tRNA modifications critical to vector survival as climate change progresses and the dynamics between tRNA expression and modifications during pathogen transmission.

项目总结 转移RNA(TRNA)是RNA中含量最丰富的形式之一，是蛋白质合成所必需的。 TRNA中的化学修饰的核糖核苷在从解码密码子到 压力耐受性。对后生动物的研究有限，特别是在食血节肢动物方面。 关于tRNAs及其修饰。这些研究的目标是提供tRNA池的特征和 食血昆虫的改良。这些变化将在血液喂养、繁殖和 在整个有机体和组织水平上交配。我们的研究得到了以下支持：1)初步结果 从有限的样本中鉴定和量化tRNA修饰，这表明存在差异 血液喂养和性别之间的关系；以及2)对以前发表的数据集的重新分析表明 与tRNA修饰相关的基因模式在血餐后和性别之间有所不同。 这项研究有两个具体目标： 具体目标1.描述昆虫在不同性别和取血过程中对转移RNA的修饰。 具体目的2.研究转移RNA修饰在血液摄食和繁殖中的作用。 在这项拟议的研究完成后，我们将建立tRNA表达和修饰图谱 多种多样的食血物种与繁殖和食血有关。几项修改的作用 将通过使用特定tRNA修饰酶的靶向还原来阐明。这些建议的研究 将是第一个广泛研究tRNA在食血昆虫中的表达和修饰模式的实验室。我们的 这些结果将为未来基于tRNA的食血昆虫的新研究提供基础，例如 随着气候变化的进展，确定对媒介生存至关重要的tRNA修饰，以及 TRNA在病原体传播过程中的表达和修饰。