tRNA modifications as critical components of insect blood feeding and reproduction

tRNA 修饰作为昆虫血液喂养和繁殖的关键组成部分

• 批准号: 10648600
• 负责人: Josh B. Benoit
• 金额: $ 20.25万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10648600
• 关键词: Area; Arthropods; Bacteria; Biochemical; Biological; Biology; Blood; Chemicals; Codon Nucleotides; Culicidae; Data; Data Set; Enzymes; Eukaryota; Event; Fat Body; Female; Future; Gene Expression Profile; Gene Expression Profiling; Generations; Genes; Genetic Transcription; Goals; Hour; Human; Insect Vectors; Insecta; Insecticide Resistance; Life; Life Cycle Stages; Link; Methods; Midgut; Modification; Nucleosides; Nutrient; Organ; Organism; Osmosis; Output; Oxidative Stress; Partner in relationship; Pattern; Phenotype; Physiological; Physiology; Play; Population; Process; Production; Protein Biosynthesis; Publishing; RNA; Reproduction; Research; Resources; Ribonucleosides; Role; Sampling; Sex Differences; Structure; System; Testing; Time; Tissue-Specific Gene Expression; Tissues; Transcript; Transfer RNA; Untranslated RNA; Whole Organism; Yeasts; climate change; disease transmission; egg; experimental study; feeding; male; novel; pathogen; prevent; reproductive; reproductive organ; response; sex; stress tolerance; transmission process; vector; vector control

Project summary Transfer RNAs (tRNA) represent one of the most abundant forms of RNA and are required for protein synthesis. Chemically modified ribonucleosides in tRNAs play critical roles in aspects that range from decoding codons to stress tolerance. Limited studies for metazoans, specifically in blood feeding arthropods, have been conducted on tRNAs and their modifications. The goal of these studies is to provide a characterization of the tRNA pool and modifications in blood feeding insects. These changes will be examined during blood feeding, reproduction, and mating at the whole organism and tissue levels. Our studies are supported by the following: 1) preliminary results from limited samples, which identified and quantified tRNA modifications, that suggest there are differences between blood feeding and sexes; and 2) Re-analysis of previously published data sets indicate expression patterns of genes associated with tRNA modification vary following a blood meal and between sexes. This study has two specific aims: Specific Aim 1. Characterize transfer RNA modifications in insects between sexes and during blood feeding. Specific Aim 2. Investigate the role of transfer RNA modifications during blood feeding and reproduction. Upon completion of this proposed research, we will establish tRNA expression and modification profiles for multiple blood feeding species in relation to reproduction and blood feeding. The role of several modifications will be elucidated by the use of targeted reduction of specific tRNA modifying enzymes. These proposed studies will be the first to extensively examine tRNA expression and modification patterns in blood feeding insects. Our results will provide the groundwork for future, novel tRNA-based studies of blood feeding insects such as identifying tRNA modifications critical to vector survival as climate change progresses and the dynamics between tRNA expression and modifications during pathogen transmission.

项目摘要 转移RNA（tRNA）代表了最丰富的RNA形式之一，是蛋白质合成所必需的。 TRNA中化学修饰的核糖核苷在从解码器到密码子到的方面起着关键作用 压力耐受性。已经进行了对后生动物的有限研究，特别是在血液喂养节肢动物中进行的研究 关于TRNA及其修改。这些研究的目的是提供tRNA池的表征， 血液喂养昆虫的修饰。这些变化将在献血，繁殖期间进行检查，并 在整个生物体和组织水平上交配。我们的研究得到以下支持：1）初步结果 从有限的样品（确定和量化的tRNA修饰）中，这表明存在差异 在血液喂养和性别之间； 2）对先前发布的数据集的重新分析表示表达 与tRNA修饰相关的基因模式在血液粉和性别之间有所不同。 这项研究有两个具体的目标： 特定目标1。表征性别和血液喂养过程中昆虫中的RNA修饰。 具体目的2。研究在血液喂养和繁殖过程中转移RNA修饰的作用。 这项提出的研究完成后，我们将建立tRNA表达和修改曲线 与生殖和血液喂养有关的多种血液喂养物种。几种修改的作用 通过使用针对特定的tRNA修饰酶的靶向减少来阐明。这些提出的研究 将是第一个在血液喂养昆虫中广泛检查tRNA表达和修饰模式的人。我们的 结果将为未来的新型基于trna的血液喂养昆虫（例如 随着气候变化的进展，识别对向量存活至关重要的tRNA修饰 病原体传播过程中的tRNA表达和修饰。