Photochemical Technologies for Improved Glycosylation Reactions

碳水化合物、多糖和它们的结合物在非常广泛的 生物过程的光谱，其中许多过程对人类健康至关重要， 发展和疾病状况。依赖多糖的生物多样性 过程反映在更加多样化的范围和复杂性上 隐含的多聚糖和糖共轭，统称为糖蛋白。这项研究 依赖于多糖的生物过程及其最终调节和/或 人类医学的开发受到分离和分离的困难的极大阻碍 从结构上表征了大量有意义的纯独特多聚糖，称为 均一的糖类，来自生物来源。因此，这样简单是势在必行的 开发实用的多糖合成技术，将稳健性和 易于操作，以便专家和非专家都能够访问 相对容易的多聚糖。这项提议的目的正是开发一种可伸缩的 解决化学合成的广泛交叉问题所需的可靠方法 糖糖的一节。重点介绍了直接光化学的发展。 方法不需要外部添加剂，从而将系统降低到其 精华-糖基供体和糖基受体-并消除了 尽最大可能。要开发的技术旨在利用 发光二极管(LED)作为光源并最终在FLOW下应用 条件，以便能够实际放大合成具有生物重要性的 多聚糖。

Carbohydrates, glycans, and their conjugates play critical roles in a very broad spectrum of biological processes, many of which are central to human health, development and disease states. The diversity of glycan-dependent biological processes is reflected by the even-more diverse spectrum and complexity of the implicated glycans and glycoconjugates, collectively known as the glycome. The study of glycan dependent biological processes and their eventual regulation and/or exploitation in human medicine is enormously hampered by difficulties in isolating and structurally characterizing meaningful quantities of pure unique glycans, known as homogeneous glycoforms, from biological sources. It is imperative therefore that simple practical technologies for glycan synthesis be developed that combine robustness with ease of operation such that specialist and non-specialists alike will be able to access glycans with relative ease. This proposal aims to develop exactly the kind of scalable robust methodology that is required to address the chemical synthesis of a broad cross section of the glycome. The focus is on the development of direct photochemical methods without the need for external additives thereby reducing the system to its essentials – a glycosyl donor and glycosyl acceptor – and eliminating complexity to the greatest extent possible. The technologies to be developed are designed to employ light-emitting diodes (LEDs) as light sources and ultimately to be applicable under flow conditions so as to enable practical scaled-up syntheses of biologically important glycans.