Photochemical Technologies for Improved Glycosylation Reactions

碳水化合物、聚糖和它们的缀合物在非常广泛的 一系列生物过程，其中许多对人类健康至关重要， 发展和疾病状态。聚糖依赖性生物学的多样性 更多样化的频谱和复杂性反映了这一进程， 所涉及的聚糖和糖缀合物，统称为糖组。研究 聚糖依赖性生物过程及其最终调节和/或 人类医学的开发受到分离和 在结构上表征有意义数量的纯独特聚糖，称为 均质糖型，来自生物来源。因此，当务之急是， 开发实用的聚糖合成技术，该技术将联合收割机的稳健性与 易于操作，使专家和非专家都能够访问 相对容易的聚糖。该提案旨在开发一种可扩展的 解决广泛交叉的化学合成所需的强大方法 糖组的一部分。重点是发展直接光化学 不需要外部添加剂的方法，从而将系统降低到其 必需品-糖基供体和糖基受体-并消除复杂性 尽最大可能。开发的技术旨在利用 发光二极管（LED）作为光源，并最终适用于流动 条件，以便能够实际按比例放大合成生物重要的 聚糖

Carbohydrates, glycans, and their conjugates play critical roles in a very broad spectrum of biological processes, many of which are central to human health, development and disease states. The diversity of glycan-dependent biological processes is reflected by the even-more diverse spectrum and complexity of the implicated glycans and glycoconjugates, collectively known as the glycome. The study of glycan dependent biological processes and their eventual regulation and/or exploitation in human medicine is enormously hampered by difficulties in isolating and structurally characterizing meaningful quantities of pure unique glycans, known as homogeneous glycoforms, from biological sources. It is imperative therefore that simple practical technologies for glycan synthesis be developed that combine robustness with ease of operation such that specialist and non-specialists alike will be able to access glycans with relative ease. This proposal aims to develop exactly the kind of scalable robust methodology that is required to address the chemical synthesis of a broad cross section of the glycome. The focus is on the development of direct photochemical methods without the need for external additives thereby reducing the system to its essentials – a glycosyl donor and glycosyl acceptor – and eliminating complexity to the greatest extent possible. The technologies to be developed are designed to employ light-emitting diodes (LEDs) as light sources and ultimately to be applicable under flow conditions so as to enable practical scaled-up syntheses of biologically important glycans.