Revealing functional networks and circuits of the posteromedial cortex withanatomical connectivity

项目摘要 后内侧皮质是大脑中的一个区域，在许多精神疾病中尤其异常， 包括抑郁症、精神分裂症和焦虑症。然而，它传统上很少受到来自 神经学家，部分原因是我们不了解其潜在的生物学。我们建议对此进行补救 在非人类灵长类动物中使用束追踪和扩散发现后内侧皮质回路的问题- 非人灵长类和人类的加权磁共振成像。这条管道将允许我们创建 根据其解剖连通性对后内侧皮质的分割。首先，我们建议将瓷砖 非人灵长类动物的后内侧皮质和束示踪剂注射，这将使我们能够分析其 与其他更好理解的大脑区域的连接。我们将使用这些数据来分割后内侧 大脑皮层根据其解剖连通性。我们期待不同的细分市场将连接到不同的， 大脑中定义明确的网络。接下来，我们将收集非人类的弥散加权神经成像数据 灵长类动物和人类。虽然这种方法允许对某些连接进行非侵入性检测，但它的 容易出错的容易出错的我们将使用轨迹追踪数据中确定的白质组织原则 来指导和调试这些神经成像数据。因为无法在人类身上进行轨迹追踪，所以这 管道提供了一种罕见的可能性来推断人脑的解剖连接。最后，基于先前的 在神经成像实验中，科学家们假设后内侧皮质可能与 尤其是大量的大脑区域和网络。因此，我们想要确定轴突的范围 这一脑区神经元的侧支作用。换句话说，单个后内侧皮质神经元 投射到许多其他大脑区域，或者这些回路在很大程度上是分开的？总之，我们期待着这些项目 将以这样一种方式阐明PMC的解剖连接，即大规模的神经成像网络可以连接 有特定的神经元回路。

Project Summary The posteromedial cortex is a brain region that is especially abnormal in many psychiatric diseases, including depression, schizophrenia, and anxiety. However, it has traditionally received little attention from neuroscientists, in part because we do not understand its underlying biology. We propose to remedy this problem by uncovering posteromedial cortical circuits using tract-tracing in nonhuman primates and diffusion- weighted magnetic resonance imaging in nonhuman primates and humans. This pipeline will allow us to create a segmentation of the posteromedial cortex according to its anatomical connectivity. First, we propose to tile the nonhuman primate posteromedial cortex with tract-tracer injections, which will allow us to analyze its connectivity with other, better-understood brain regions. We will use these data to segment the posteromedial cortex according to its anatomical connectivity. We expect that different segments will connect with distinct, well-defined networks within the brain. Next, we will collect diffusion-weighted neuroimaging data in nonhuman primates and humans. Although this method allows noninvasive detection of some connections, it is highly susceptible to errors. We will use the principles of white matter organization identified in the tract-tracing data to guide and debug these neuroimaging data. Because tract-tracing cannot be performed in humans, this pipeline offers a rare possibility to infer anatomical connectivity in the human brain. Finally, based on prior neuroimaging experiments, scientists have hypothesized that the posteromedial cortex may connect with a particularly large number of brain regions and networks. Thus, we would like to determine the extent of axonal collateralization in the neurons of this brain region. In other words, does a single posteromedial cortical neuron project to many other brain regions, or are the circuits largely separate? Together, we expect these projects will elucidate PMC anatomical connectivity in such a way that large-scale neuroimaging networks can be linked with specific neuronal circuits.