Regulation of DNA Replication in S. Pombe

粟酒裂殖酵母 DNA 复制的调控

• 批准号: 7989658
• 负责人: THOMAS J. KELLY
• 金额: $ 12.57万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-25 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7989658
• 关键词: Animal Model; Area; Biochemical; Biological Models; Cell Cycle; Cell Proliferation; Cells; Complement; Complex; DNA Damage; DNA biosynthesis; DNA damage checkpoint; DNA replication origin; Eukaryota; Fission Yeast; Genetic; Genome Stability; Goals; Grant; Investigation; Laboratories; Methods; Molecular; Pathway interactions; Play; Property; Protein Kinase; Proteins; Regulation; Replication Initiation; Replication Origin; Research; Role; Saccharomyces cerevisiae; Work; Xenopus; checkpoint kinase 2; insight; programs; response; ultraviolet irradiation

DESCRIPTION (provided by applicant): The long-term goal of this research program is to understand how eukaryotic DMA replication is regulated during the cell cycle. This problem is central to understanding the mechanisms that control cellular proliferation and maintain genomic stability. Our approach to this problem involves the use of genetic and biochemical methods to identify and characterize proteins involved in initiation of DMA replication and its control in the model organism Schizosaccharomyces pombe. Our major objectives are to characterize the assembly and activation of initiation complexes, to define the biochemical mechanisms involved in the replication checkpoint, and to characterize a DNA replication-dependent DMA damage checkpoint that we have recently discovered. We expect that information obtained in our studies will complement that obtained from the study of the Saccharomyces cerevisiae and will be directly relevant to the control of DNA replication in higher eukaryotes.

描述（由申请人提供）：本研究计划的长期目标是了解真核生物在细胞周期中如何调节DMA复制。这个问题对于理解控制细胞增殖和维持基因组稳定性的机制至关重要。我们解决这个问题的方法包括使用遗传和生化方法来鉴定和表征模式生物裂糖菌中参与DMA复制起始及其控制的蛋白质。我们的主要目标是表征起始复合物的组装和激活，定义与复制检查点相关的生化机制，并表征我们最近发现的DNA复制依赖的DMA损伤检查点。我们期望在我们的研究中获得的信息将补充从酿酒酵母研究中获得的信息，并将与高等真核生物DNA复制的控制直接相关。

项目成果

Coordination of DNA damage tolerance mechanisms with cell cycle progression in fission yeast.

• DOI: 10.1080/15384101.2015.1121353
• 发表时间: 2016
• 期刊: Cell cycle (Georgetown, Tex.)
• 作者: Callegari AJ;Kelly TJ
• 通讯作者: Kelly TJ