Regulation of DNA Replication in S. Pombe

描述(由申请人提供)：该研究计划的长期目标是了解真核细胞的DMA复制在细胞周期中是如何调节的。这个问题对于理解控制细胞增殖和维持基因组稳定性的机制至关重要。我们解决这个问题的方法包括使用遗传和生化方法来鉴定和表征模式生物裂殖酵母中参与DNA复制启动及其控制的蛋白质。我们的主要目标是表征起始复合体的组装和激活，定义复制检查点所涉及的生化机制，并表征我们最近发现的依赖于DNA复制的DMA损伤检查点。我们希望在我们的研究中获得的信息将补充从酿酒酵母研究中获得的信息，并将与高等真核生物中DNA复制的控制直接相关。

DESCRIPTION (provided by applicant): The long-term goal of this research program is to understand how eukaryotic DMA replication is regulated during the cell cycle. This problem is central to understanding the mechanisms that control cellular proliferation and maintain genomic stability. Our approach to this problem involves the use of genetic and biochemical methods to identify and characterize proteins involved in initiation of DMA replication and its control in the model organism Schizosaccharomyces pombe. Our major objectives are to characterize the assembly and activation of initiation complexes, to define the biochemical mechanisms involved in the replication checkpoint, and to characterize a DNA replication-dependent DMA damage checkpoint that we have recently discovered. We expect that information obtained in our studies will complement that obtained from the study of the Saccharomyces cerevisiae and will be directly relevant to the control of DNA replication in higher eukaryotes.