RapidFire mass spectrometry system

RapidFire 质谱系统

• 批准号: 8826541
• 负责人: Mohit Jain
• 金额: $ 60万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-17 至 2016-04-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826541
• 关键词: Atherosclerosis; Cells; Communities; Coupled; Coupling; DNA Sequence; Development; Diabetes Mellitus; Diet; Discipline; Disease; Early Diagnosis; Environmental Exposure; Human; Human Characteristics; Individual; Kidney Diseases; Liquid substance; Mass Spectrum Analysis; Measurement; Measures; Messenger RNA; Metabolic; Metabolism; Organism; Pathogenesis; Pharmaceutical Preparations; Phase; Phenotype; Physiology; Population; Postdoctoral Fellow; Proteins; Regulation; Robotics; Sampling; Solid; System; Therapeutic Agents; Tissues; Training; Variant; graduate student; human disease; instrument; metabolomics; response; small molecule

 DESCRIPTION (provided by applicant): Metabolomics describes the systematic and comprehensive measure of small molecule metabolites (<1000 Da) present in biofluids, cells, tissues and organisms. Given the close coupling between physiology and metabolism, metabolomics holds the power to deeply phenotype humans - integrating DNA sequence variation, mRNA species levels, protein levels, environmental exposures, diet, and commensal host micro- organisms - all within a single global measurement. Such metabolomics measures allows for understanding the basic pathogenesis of human disease, as well as diagnosing of early disease, characterization of disease sub-states, and predicting drug responsiveness. Given the integrative and dynamic nature of the human circulating metabolome, high-throughput approaches that allow for assessment of metabolomics on population level scales (>100 to 1000 samples) are paramount. Recently, developments in robotic liquid handling and solid-phase extraction systems, coupled with advanced mass spectrometry now enable the high-throughput interrogation of the metabolome across tens of thousands of individuals. This proposal is for a RapidFire mass spectrometry system, to be used for the high-throughput characterization of the human metabolome in healthy and disease states. This instrument will be employed for understanding the metabolic determinants of diseases such as diabetes, renal disease and atherosclerosis; the response to therapeutic agents, such as statins; and probing the metabolic regulation of commensal micro-organisms. Importantly, this RapidFire instrument will be used for the study of humans and human samples, which are not possible using currently available approaches. In addition, this instrument will be used to engage the scientific community in metabolomics and for training of graduate students and postdoctoral fellows in the emerging discipline of metabolomics.



项目成果

Substantial Fat Loss in Physique Competitors Is Characterized by Increased Levels of Bile Acids, Very-Long Chain Fatty Acids, and Oxylipins.

• DOI: 10.3390/metabo12100928
• 发表时间: 2022-09-30
• 期刊: Metabolites
• 影响因子: 4.1
• 作者: Sarin HV;Hulmi JJ;Qin Y;Inouye M;Ritchie SC;Cheng S;Watrous JD;Nguyen TC;Lee JH;Jin Z;Terwilliger JD;Niiranen T;Havulinna A;Salomaa V;Pietiläinen KH;Isola V;Ahtiainen JP;Häkkinen K;Jain M;Perola M
• 通讯作者: Perola M