High dynamic range multiphoton microscopy for large-scale imaging

摘要 多光子显微镜是高分辨率功能脑的首选技术之一 由于其在厚组织中具有显著的深度穿透性，因此可用于成像。在标准配置中， 这种成像包括在整个样本中以3D方式扫描飞秒激光焦点。这个 激光功率在扫描过程中是固定的，图像信息包含在时间依赖关系中 检测到的荧光信号。这项技术可能会出现几个问题。首先，在 常见的情况下，样本包含极端变化的亮度，例如 在大型SoMAS和更精细的树枝状过程之间，通常不可能捕捉到 全范围信号，而不会在扫描明亮区域时使探测器饱和，或者 在昏暗区域扫描时失去信号。第二，当成像来自于 GCaMP等功能记者，出现无法预测的较大亮度变化 提前，迫使用户使用低照度以最大限度地减少探测器的可能性 饱和，因此可能会影响信噪比。第三，在执行体积扫描时 通过扩大深度范围，单个激光功率要么变得太弱，要么变得太大 深度或在浅层太强。 我们提出了一个简单的解决方案来解决所有这些问题。解决方案涉及到积极的 使用反馈电子学逐个像素地调节激光功率。我们已经证明了 我们的技术可以将双光子显微镜的动态范围提高几个数量级 中快像素量级为20S，实现了前所未有的高动态 范围(HDR)为1011：1。我们对此项目的目标是： 1)开发用于视频率HDR成像的超快反馈电子设备。 2)用于大范围多区域HDR成像的开关多路复用技术的发展。 3)多光子HDR成像在小鼠脑解剖和功能成像中的应用。 我们的目标是实现全面的大规模多光子成像，具有前所未有的 以一种简单的方式提供动态范围，许多实验室都可以轻松实施，网址为 合理的成本和最小的硬件修改。

ABSTRACT Multiphoton microscopy is one of the preferred techniques for high-resolution functional brain imaging because of its remarkable depth penetration in thick tissue. In standard configurations, such imaging involves scanning a femtosecond laser focus in 3D throughout a sample. The laser power is fixed during the scan and image information is contained in the time dependence of the detected fluorescence signal. Several problems can occur with this technique. First, in common cases where the sample contains extreme variations in brightness, for example between large somas and much finer dendritic processes, it is often impossible to capture the full range of signals without either saturating the detector when scanning over bright regions, or losing signal when scanning over dim regions. Second, when imaging time-varying signals from functional reporters such as GCaMP, large brightness variations occur that cannot be predicted in advance, forcing the user to use a low illumination to minimize the possibility of detector saturation, thus potentially compromising SNR. Third, when performing volumetric scans through an extended range of depths, a single laser power becomes either too weak at large depths or too strong at shallow depths. We propose a simple solution to solve all these problems. The solution involves actively regulating the laser power pixel-by-pixel using feedback electronics. We have demonstrated that our technique can improve the dynamic range of two-photon microscopes by several orders of magnitude for moderately fast pixel times of 20s, achieving an unprecedentedly high dynamic range (HDR) of 1011:1. Our goals for this project are the: 1) Development of ultrafast feedback electronics for video-rate HDR imaging. 2) Development of switched multiplexing technique for large-scale multi-region HDR imaging. 3) Application of multiphoton HDR imaging to anatomical and functional mouse brain imaging. Our goal is to enable comprehensive large-scale multiphoton imaging with unprecedented dynamic range in a simple manner that can be readily implementable by many labs at reasonable cost and with minimal hardware modifications.