DISSEMINATION 274-320

项目摘要/摘要 位于麦迪逊的国家磁共振设施(NMRFAM)是生物分子的资源中心 核磁共振波谱和小角X射线散射。NMRFAM的目标是 以资源技术和开发拓展生物分子核磁共振波谱前沿 程序在重要领域(1)高通量确定较小的结构和功能 蛋白质和RNA分子，(2)研究挑战的结构和动力学的技术 系统，如复合体、膜蛋白、顺磁性蛋白和更大的RNA分子，以及(3) 代谢组学的有效方法，筛选与生物大分子结合的小分子，以及 天然产物分析。NMRFAM努力成为更大生物界的示范 核磁共振波谱的尖端能力。以扩大其科学活动范围为目标， NMRFAM接待了与其研究技术相关领域工作的杰出来访科学家 发展项目。通过其合作活动，NMRFAM开发和传播先进的 涵盖生物分子核磁共振研究的所有步骤的方法。该中心提供从头到尾的支持 在以下一个或多个步骤中协助进行生物医学核磁共振研究：(1)策略 评价和实验设计，(2)蛋白质和核酸的制备和标记，(3)可行性 研究，(4)数据收集，(5)数据分析和结构确定，(6)数据存储，和(7) 手稿准备工作。NMRFAM旨在通过提供 拥有与其特定需求相匹配的资源的研究人员。一个主要的目标是开发制作 这些研究速度更快、成本更低，并且适用于更大类别的蛋白质和核酸 对人类健康很重要。NMRFAM为年轻的研究人员和经验丰富的光谱学家提供 访问最先进的仪器，支持多种数据收集模式，无论是作为服务还是 协作项目。通过开发的协议、脉冲序列、软件工具和数据库 NMRFAM的研究活动向普通科学界提供。用户可以亲身体验 在中心接受训练。NMRFAM举办年度研讨会和小组培训课程，作为 培训其用户基础并传播其新技术。用于传播的其他机制 NMRFAM技术包括通讯、NMRFAM网站、软件服务器、代谢组学数据库 (在BMRB主持)，在会议上发言，以及发表文章和评论。

PROJECT SUMMARY/ABSTRACT The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource center for biomolecular nuclear magnetic resonance (NMR) spectroscopy and small angle X-ray scattering (SAXS). NMRFAM aims to expand the frontiers of biomolecular NMR spectroscopy through resource technology and development programs in the important areas of (1) high-throughput determination of structures and functions of smaller proteins and RNA molecules, (2) technology for investigating the structure and dynamics of challenging systems, such as complexes, membrane proteins, paramagnetic proteins, and larger RNA molecules, and (3) efficient approaches to metabolomics, screening of small molecules binding to biological macromolecules, and natural product analysis. NMRFAM strives to be a model to the larger biological community for demonstrating cutting-edge capabilities of NMR spectroscopy. With the goal of broadening the scope of its scientific activities, NMRFAM hosts distinguished visiting scientists working in areas related to its research technology development projects. Through its collaborative activities, NMRFAM develops and disseminates advanced approaches that cover all steps in biomolecular NMR investigations. The center offers start-to-finish support for biomedical NMR investigations with assistance in one or more of the following steps: (1) strategy evaluation and experiment design, (2) preparation and labeling of proteins and nucleic acids, (3) feasibility studies, (4) data collection, (5) data analysis and structure determination, (6) data deposition, and (7) manuscript preparation. NMRFAM aims to facilitate the efficient pursuit of new knowledge by providing researchers with resources matched to their particular needs. A major goal is to develop methods for making these investigations faster and less costly as well as applicable to larger classes of proteins and nucleic acids of importance in human health. NMRFAM provides young investigators and experienced spectroscopists access to state-of-the-art instrumentation with support for multiple modes of data collection either as service or collaborative projects. Protocols, pulse sequences, software tools, and databases developed through NMRFAM’s research activities are made available to the general scientific community. Users receive hands-on training at the center. NMRFAM conducts annual workshops and group training sessions as other means for training its user base and for disseminating its novel technology. Additional mechanisms used to disseminate NMRFAM technology include newsletters, the NMRFAM website, software servers, a metabolomics database (hosted at BMRB), presentations at meetings, and the publication of articles and reviews.