DISSEMINATION 274-320

项目总结/摘要 位于麦迪逊的国家磁共振设施（NMRFAM）是生物分子的资源中心。 核磁共振（NMR）光谱和小角X射线散射（SAXS）。NMRFAM旨在 通过资源技术和开发扩展生物分子NMR光谱学的前沿 方案的重要领域有（1）高通量测定结构和功能较小的 蛋白质和RNA分子，（2）研究具有挑战性的结构和动力学的技术 系统，如复合物、膜蛋白、顺磁性蛋白和较大的RNA分子，以及（3） 代谢组学的有效方法，筛选与生物大分子结合的小分子， 天然产物分析NMRFAM努力成为更大的生物界的典范， 核磁共振光谱学的尖端能力。为了扩大其科学活动的范围， NMRFAM主办杰出的访问科学家在其研究技术相关领域的工作 发展项目。通过其合作活动，NMRFAM开发和传播先进的 这些方法涵盖了生物分子NMR研究的所有步骤。该中心提供从开始到结束的支持 对于生物医学NMR研究，在以下一个或多个步骤中提供帮助：（1）策略 评价和实验设计，（2）蛋白质和核酸的制备和标记，（3）可行性 研究，（4）数据收集，（5）数据分析和结构确定，（6）数据存储，以及（7） 手稿准备。NMRFAM旨在通过提供以下服务来促进对新知识的有效追求 研究人员的资源与他们的特殊需求相匹配。一个主要的目标是开发方法， 这些研究速度更快，成本更低，适用于更大类别的蛋白质和核酸 对人类健康的重要性。NMRFAM提供年轻的研究人员和经验丰富的光谱学家 访问最先进的仪器，支持多种数据收集模式， 合作项目。协议，脉冲序列，软件工具和数据库开发，通过 国家海洋研究和渔业管理局的研究活动向广大科学界开放。用户获得动手操作 在中心训练。作为其他手段， 培训其用户群和传播其新技术。用于传播的其他机制 NMRFAM技术包括通讯，NMRFAM网站，软件服务器，代谢组学数据库 （由BMRB主办），在会议上发言，发表文章和评论。

PROJECT SUMMARY/ABSTRACT The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource center for biomolecular nuclear magnetic resonance (NMR) spectroscopy and small angle X-ray scattering (SAXS). NMRFAM aims to expand the frontiers of biomolecular NMR spectroscopy through resource technology and development programs in the important areas of (1) high-throughput determination of structures and functions of smaller proteins and RNA molecules, (2) technology for investigating the structure and dynamics of challenging systems, such as complexes, membrane proteins, paramagnetic proteins, and larger RNA molecules, and (3) efficient approaches to metabolomics, screening of small molecules binding to biological macromolecules, and natural product analysis. NMRFAM strives to be a model to the larger biological community for demonstrating cutting-edge capabilities of NMR spectroscopy. With the goal of broadening the scope of its scientific activities, NMRFAM hosts distinguished visiting scientists working in areas related to its research technology development projects. Through its collaborative activities, NMRFAM develops and disseminates advanced approaches that cover all steps in biomolecular NMR investigations. The center offers start-to-finish support for biomedical NMR investigations with assistance in one or more of the following steps: (1) strategy evaluation and experiment design, (2) preparation and labeling of proteins and nucleic acids, (3) feasibility studies, (4) data collection, (5) data analysis and structure determination, (6) data deposition, and (7) manuscript preparation. NMRFAM aims to facilitate the efficient pursuit of new knowledge by providing researchers with resources matched to their particular needs. A major goal is to develop methods for making these investigations faster and less costly as well as applicable to larger classes of proteins and nucleic acids of importance in human health. NMRFAM provides young investigators and experienced spectroscopists access to state-of-the-art instrumentation with support for multiple modes of data collection either as service or collaborative projects. Protocols, pulse sequences, software tools, and databases developed through NMRFAM’s research activities are made available to the general scientific community. Users receive hands-on training at the center. NMRFAM conducts annual workshops and group training sessions as other means for training its user base and for disseminating its novel technology. Additional mechanisms used to disseminate NMRFAM technology include newsletters, the NMRFAM website, software servers, a metabolomics database (hosted at BMRB), presentations at meetings, and the publication of articles and reviews.