DISSEMINATION 274-320

传播274-320

• 批准号: 9133407
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 7.32万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9133407
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PROJECT SUMMARY/ABSTRACT The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource center for biomolecular nuclear magnetic resonance (NMR) spectroscopy and small angle X-ray scattering (SAXS). NMRFAM aims to expand the frontiers of biomolecular NMR spectroscopy through resource technology and development programs in the important areas of (1) high-throughput determination of structures and functions of smaller proteins and RNA molecules, (2) technology for investigating the structure and dynamics of challenging systems, such as complexes, membrane proteins, paramagnetic proteins, and larger RNA molecules, and (3) efficient approaches to metabolomics, screening of small molecules binding to biological macromolecules, and natural product analysis. NMRFAM strives to be a model to the larger biological community for demonstrating cutting-edge capabilities of NMR spectroscopy. With the goal of broadening the scope of its scientific activities, NMRFAM hosts distinguished visiting scientists working in areas related to its research technology development projects. Through its collaborative activities, NMRFAM develops and disseminates advanced approaches that cover all steps in biomolecular NMR investigations. The center offers start-to-finish support for biomedical NMR investigations with assistance in one or more of the following steps: (1) strategy evaluation and experiment design, (2) preparation and labeling of proteins and nucleic acids, (3) feasibility studies, (4) data collection, (5) data analysis and structure determination, (6) data deposition, and (7) manuscript preparation. NMRFAM aims to facilitate the efficient pursuit of new knowledge by providing researchers with resources matched to their particular needs. A major goal is to develop methods for making these investigations faster and less costly as well as applicable to larger classes of proteins and nucleic acids of importance in human health. NMRFAM provides young investigators and experienced spectroscopists access to state-of-the-art instrumentation with support for multiple modes of data collection either as service or collaborative projects. Protocols, pulse sequences, software tools, and databases developed through NMRFAM’s research activities are made available to the general scientific community. Users receive hands-on training at the center. NMRFAM conducts annual workshops and group training sessions as other means for training its user base and for disseminating its novel technology. Additional mechanisms used to disseminate NMRFAM technology include newsletters, the NMRFAM website, software servers, a metabolomics database (hosted at BMRB), presentations at meetings, and the publication of articles and reviews.

项目概要/摘要 麦迪逊国家磁共振设施 (NMRFAM) 是生物分子资源中心 核磁共振 (NMR) 光谱和小角 X 射线散射 (SAXS)。 NMRFAM 旨在 通过资源技术和开发拓展生物分子核磁共振波谱学的前沿 重要领域的计划 (1) 较小结构和功能的高通量测定 蛋白质和RNA分子，（2）研究挑战性结构和动力学的技术 系统，例如复合物、膜蛋白、顺磁性蛋白和较大的 RNA 分子，以及 (3) 代谢组学的有效方法，筛选与生物大分子结合的小分子，以及 天然产物分析。 NMRFAM 致力于成为更大的生物界的典范，以展示 核磁共振波谱的尖端能力。为了扩大其科学活动的范围， NMRFAM 接待了在其研究技术相关领域工作的杰出访问科学家 开发项目。通过其协作活动，NMRFAM 开发并传播了先进的 涵盖生物分子 NMR 研究所有步骤的方法。该中心提供从始至终的支持 在以下一个或多个步骤的协助下进行生物医学 NMR 研究： (1) 策略 评估和实验设计，（2）蛋白质和核酸的制备和标记，（3）可行性 研究，(4) 数据收集，(5) 数据分析和结构确定，(6) 数据沉积，以及 (7) 手稿准备。 NMRFAM 旨在通过提供 研究人员拥有与其特定需求相匹配的资源。一个主要目标是开发制造方法 这些研究速度更快、成本更低，并且适用于更大类别的蛋白质和核酸 对人类健康具有重要意义。 NMRFAM 提供年轻的研究人员和经验丰富的光谱学家 访问最先进的仪器，支持多种数据收集模式，无论是作为服务还是 合作项目。通过开发协议、脉冲序列、软件工具和数据库 NMRFAM 的研究活动向广大科学界开放。用户可亲身体验 在中心进行培训。 NMRFAM 举办年度研讨会和团体培训课程，作为其他方式 培训其用户群并传播其新技术。用于传播的附加机制 NMRFAM 技术包括时事通讯、NMRFAM 网站、软件服务器、代谢组学数据库 （在 BMRB 主办）、会议上的演讲以及文章和评论的出版。