MRI Toolbox for Rodent Brain Microstructure Imaging

用于啮齿动物脑微结构成像的 MRI 工具箱

• 批准号: 9026974
• 负责人: MARK D DOES
• 金额: $ 42.56万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2019-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9026974
• 关键词: Algorithms; Animal Model; Animals; Axon; Biological Assay; Brain; Brain region; C57BL/6 Mouse; Caliber; Calibration; Cerebrum; Communities; Computer software; Contrast Media; Data; Dependence; Disease; Electron Microscopy; Evaluation; Gadolinium; Health; Histology; Image; Image Analysis; Imaging Device; Imaging Phantoms; Imaging technology; Injury; Instruction; Location; Magnetic Resonance Imaging; Magnetism; Maps; Measurement; Methods; Modality; Modeling; Morphologic artifacts; Mus; Myelin; Neurons; Performance; Physiologic pulse; Process; Property; Protocols documentation; Rattus; Relaxation; Research; Research Personnel; Resolution; Rodent; Running; Scanning; Scientist; Series; Site; Slice; Software Tools; Sprague-Dawley Rats; System; Testing; Time; Tissues; Variant; Vendor; Water; Weight; Work; base; cost; design; gadolinium oxide; human disease; image reconstruction; imaging modality; in vivo; indexing; insight; interest; mouse model; novel; open source; platform-independent; programs; quality assurance; rate of change; research study; tool; white matter

DESCRIPTION (provided by applicant): The overarching aim of this proposal is to develop and disseminate magnetic resonance imaging (MRI) tools to identify and characterize neuronal microstructure in small animals. MRI is an ideal modality for such studies because it can be applied non-invasively in vivo or non-destructively ex vivo, and can provide 3D evaluation across the entire brain. We will develop and test standardized MRI pulse sequences across multiple vendor platforms and develop an easily produced and standardized quality-assurance phantom and associated imaging protocol. Automated and optimized image reconstruction and quantitative microstructural parameter mapping software will be developed and disseminated as open source. A rigorous algorithmic framework for planning statistically-optimized, multi-objective imaging protocols will be developed and implemented in open source multi-platform software. A series of experimental studies will establish probabilistic volumes of a variety of neuronal microstructural property maps in normal and abnormal rat and mouse brains, along with quantitatively evaluated histology of select cerebral white matter regions. Throughout the project, novel MRI methods will be developed and tested, a variety of state-of-the-art methods will be standardized and optimized, and comparisons of MRI data with histology will offer novel insights in the micro anatomical basis of MRI contrast in white matter.

描述（由申请人提供）：该提案的总体目标是开发和传播磁共振成像（MRI）工具来识别和表征小动物的神经元微观结构。 MRI 是此类研究的理想方式，因为它可以非侵入性体内或非破坏性离体应用，并且可以提供整个大脑的 3D 评估。我们将跨多个供应商平台开发和测试标准化 MRI 脉冲序列，并开发易于生产和标准化的质量保证体模和相关成像协议。自动化和优化的图像重建和定量微观结构参数映射软件将作为开源开发和传播。将在开源多平台软件中开发和实施用于规划统计优化的多目标成像协议的严格算法框架。一系列实验研究将建立正常和异常大鼠和小鼠大脑中各种神经元微观结构特性图的概率体积，以及对选定脑白质区域的定量评估组织学。在整个项目中，将开发和测试新的 MRI 方法，对各种最先进的方法进行标准化和优化，并将 MRI 数据与组织学进行比较，将为白质 MRI 对比的微观解剖基础提供新的见解。