An ultricentrifuge : a basic instrument for the isolation of clathrin-coated vesicles by subcellular fractionation in order to do functional proteomic analysis

超速离心机：通过亚细胞分级分离网格蛋白包被的囊泡以进行功能蛋白质组分析的基本仪器

• 批准号: 344932-2007
• 负责人: LegendreGuillemin, Valérie
• 金额: $ 5.46万
• 依托单位: Université du Québec à Chicoutimi
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326037
• 关键词: ultricentrifuge; basic; instrument; isolation; clathrin

The University of Quebec at Chicoutimi (UQAC) is developping cell biology research. Cell biology studies the structure and the function of cells. It is important to study the function of cells since there are the major part of our body. Their localization and their function are highly regulated. Change in their organization could lead to a dysregulation of the system and then to the development of diseases. Inside the cells, there are different compartments containing protein complexes involved in cell function. Techniques have been developped such as compartment isolation to study these compartments and protein complexes in order to better understand protein and cell functions. One of the technique is the ultracentrifugation that allow to isolate specific compartments and to study their protein contents. In order to establish my research program at UQAC, I need to acquire an ultracentrifuge since this is a new field of investigation at UQAC and there is no such equipment available.

魁北克大学希库蒂米分校 (UQAC) 正在开展细胞生物学研究。细胞生物学研究细胞的结构和功能。研究细胞的功能非常重要，因为细胞是我们身体的主要部分。它们的定位和功能受到严格监管。组织的变化可能会导致系统失调，进而导致疾病的发生。细胞内部有不同的区室，其中含有参与细胞功能的蛋白质复合物。已经开发出隔室分离等技术来研究这些隔室和蛋白质复合物，以便更好地了解蛋白质和细胞功能。其中一项技术是超速离心，可以分离特定的区室并研究其蛋白质含量。为了在 UQAC 建立我的研究项目，我需要购买一台超速离心机，因为这是 UQAC 的一个新研究领域，并且没有此类设备。