Histology processing equipment

组织学处理设备

• 批准号: 344953-2007
• 负责人: Kerr, Leslie
• 金额: $ 4.79万
• 依托单位: Trent University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326192
• 关键词: Histology; processing; equipment

A tissue processor is an essential piece of equipment to support Dr. Kerr's research, currently funded by an NSERC Discovery grant, which examines the effects of neonatal care-giving environment on gene and protein expression within the context of normal and malignant mammary gland development.  In addition, to Dr. Kerr, several other Trent University faculty members (NSERC holders or NSERC applicants) rely on the reliable use of a tissue processor.  For example, Dr. Craig Brunetti's (co-applicant on this RTI-1) research focus involves the characterization of genes involved in viral pathogenesis, whereas Dr. C. Kapron's research examines protein and gene expression changes in cellular stress signalling pathways in developing mammalian embryos due to environmental toxin exposure.  An essential part of isolating possible factors that are involved in gene expression and which represent the complex interplay between the animal and its environment is immunohistochemical examination of organs or whole organisms. The ability to identify exactly where in an organ or animal protein expression is altered due to social, viral, or toxicological environmental exposures will help to explain, in combination with other forms of assessment, the effects of that particular environmental factor on animal health, pathology, as well as basic physiological functions.  Immunohistochemistry remains a fundamental technique that provides an important biological complement to current cutting-edge techniques including tissue micorarrays and functional genomics.  Without this infrastructure, which is currently not available at Trent University, Dr.s Kerr's and Brunetti's research programs are severely restricted in the types of developmental and in vivo questions that can be addressed.

组织处理器是支持Kerr博士研究的重要设备，目前由NSERC发现基金资助，该基金研究了新生儿护理环境对正常和恶性乳腺发育背景下基因和蛋白质表达的影响。此外，对于Kerr博士，特伦特大学的其他几位教师（NSERC持有者或NSERC申请者）依赖于组织处理器的可靠使用。例如，克雷格布鲁内蒂博士（RTI-1的共同申请人）的研究重点涉及病毒发病机制中涉及的基因的表征，而C。Kapron的研究检测了由于环境毒素暴露而导致的哺乳动物胚胎发育中细胞应激信号通路中蛋白质和基因表达的变化。分离参与基因表达的可能因素以及代表动物与其环境之间复杂相互作用的重要部分是器官或整个生物体的免疫组织化学检查。准确识别器官或动物蛋白质表达因社会、病毒或毒理学环境暴露而改变的位置的能力将有助于结合其他形式的评估来解释该特定环境因素对动物健康、病理学、免疫组织化学仍然是一种基本的技术，为目前的生物学功能提供了重要的生物学补充。尖端技术，包括组织微阵列和功能基因组学。没有这种基础设施，这是目前在特伦特大学，博士s克尔和布鲁内蒂的研究计划是严重限制在类型的发展和在体内的问题，可以解决。