Cell surface protein antigens of Porphyromonas gingivalis

牙龈卟啉单胞菌细胞表面蛋白抗原

• 批准号: nhmrc : 114203
• 负责人: Prof Eric Reynolds
• 金额: $ 22.65万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2000
• 资助国家: 澳大利亚
• 起止时间: 2000-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/cell-surface-protein-porphyromonas-gingivalis/100613
• 关键词: Cell; surface; protein; antigens; Porphyromonas

Periodontitis is a bacterial-associated disease of the supporting structures of the teeth and can result in tooth loss. The disease is classified as a major public health problem with an enormous economic burden. A bacterium Porphyromonas gingivalis has now been identified as a major causative agent of periodontitis in adults. We have developed methods to rapidly identify all the surface protein antigens of P. gingivalis using mass spectrometry and then using recombinant DNA technology to express these recombinant antigens for testing in animal models of disease. The aim of this project is: (i) to rapidly identify cell surface protein antigens of P. gingivalis using novel techniques. (ii) prepare these antigens using recombinant DNA technology. (iii) use patient and healthy subject sera as well as animal antisera to screen the recombinant antigens for suitable candidates for an immunodiagnostic product and-or vaccine. (iv) test the recombinant antigens in animal models of disease in an approach to identify a candidate antigen for a vaccine. The expected outcomes of this research are that (i) we will develop techniques that can be used to characterise the cell surface antigens of any pathogenic bacterium. (ii) we will determine the organisation and the structure of the cell surface proteins of P. gingivalis. (iii) we will identify an abundant, unique and immunogenic protein of the P. gingivalis surface that will be a suitable candidate antigen for the development of a sensitive and specific immunodiagnostic. (iv) we will identify a cell surface antigen that when used as a vaccine in animals prevents disease caused by P. gingivalis.

牙周炎是一种与细菌相关的牙齿支撑结构疾病，可导致牙齿脱落。该病被列为具有巨大经济负担的重大公共卫生问题。牙龈卟啉单胞菌现在已被确定为成人牙周炎的主要病原体。我们发展了一种方法，利用质谱仪快速鉴定牙龈假单胞菌的所有表面蛋白抗原，然后利用重组DNA技术表达这些重组抗原，用于疾病动物模型的测试。本项目的目的是：(I)利用新技术快速鉴定牙龈假单胞菌的细胞表面蛋白抗原。(Ii)利用重组DNA技术制备这些抗原。(3)使用患者和健康受试者血清以及动物抗血清来筛选重组抗原，以寻找免疫诊断产品和/或疫苗的合适候选者。(4)在疾病动物模型中测试重组抗原，以确定疫苗的候选抗原。这项研究的预期结果是：(I)我们将开发可用于表征任何病原体的细胞表面抗原的技术。(Ii)我们会确定牙龈假单胞菌细胞表面蛋白的组织和结构。(Iii)我们将在牙龈假单胞菌表面鉴定一种丰富、独特的免疫原性蛋白，该蛋白将成为一种合适的候选抗原，用于开发敏感和特异的免疫诊断方法。(Iv)我们会找出一种细胞表面抗原，在动物身上用作疫苗时，可预防牙龈假单胞菌引起的疾病。