Evolution of regulatory mechanisms controlling distal-less-related homeobox gene expression

控制远端相关同源盒基因表达的调控机制的演变

• 批准号: 121795-2006
• 负责人: Ekker, Marc
• 金额: $ 3.72万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=337383
• 关键词: Evolution; regulatory; mechanisms; controlling; distal

My laboratory studies a family of genes, the Dlx genes, which play important regulatory roles during development of the embryos of a large number of animal species.  They are parts of genetic cascades taking place during development to determine the different cell types and the patterning of the embryo, notably in the branchial arches, embryonic structures that give rise to skeleton of the face, in specific parts of the brain, in the ears, in the olfactory organs and in the limbs. We have identified the parts of the Dlx genes that enable them to function in specific parts of the embryo during development.  We showed that these gene fragments, called enhancers, can function properly not only in the species where they have been identified (e.g. mouse) but also in distantly related animals (e.g. fish).  We now want to determine when the enhancers were acquired during evolution of vertebrates to confer novel functions to the Dlx genes. For this, we are studying the lamprey, a jawless vertebrate. Lampreys have Dlx genes and they are expressed in the mouth area. Our hypothesis is that changes in the patterns of expression of Dlx genes were instrumental in the evolution of the jaw apparatus during vertebrate evolution. This research will enable us to understand how genes acquire new functions during evolution, thus participating in the emergence of morphological and functional innovations. Our research will also help us better understand how genes control the morphology of the developing embryo.

我的实验室研究一个基因家族，Dlx基因，它在许多动物物种的胚胎发育过程中发挥重要的调节作用。它们是发育过程中发生的遗传级联的一部分，以确定不同的细胞类型和胚胎的模式，特别是在鳃弓，胚胎结构，产生面部骨骼，在大脑的特定部位，耳朵，嗅觉器官和四肢我们已经确定了Dlx基因的部分，使它们能够在发育过程中在胚胎的特定部位发挥作用。我们发现，这些基因片段，称为增强子，不仅能在它们被发现的物种中正常发挥作用（例如小鼠），但也在远亲动物中我们现在想要确定在脊椎动物的进化过程中何时获得增强子以赋予Dlx基因新的功能。为此，我们正在研究七鳃鳗，一种无颌脊椎动物。七鳃鳗有Dlx基因，它们在口腔区域表达。我们的假设是，Dlx基因的表达模式的变化是在脊椎动物进化过程中的颌骨装置的演变。这项研究将使我们能够了解基因如何在进化过程中获得新的功能，从而参与形态和功能创新的出现。我们的研究还将帮助我们更好地了解基因如何控制发育中胚胎的形态。