Characterising the topology and function of the human m5C RNA methylome

表征人类 m5C RNA 甲基化组的拓扑结构和功能

• 批准号: nhmrc : 1061551
• 负责人: Prof Thomas Preiss
• 金额: $ 40.18万
• 依托单位: Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/characterising-topology-function-rna-methylome/454908
• 关键词: Characterising; topology; function; human; m5C

The role of the modified base 5-methylcytosine (m5C) as an epigenetic mark in DNA is well appreciated and intensely studied. By comparison, the cellular functions of the same base modification in RNA molecules, which function as working copies of the DNA genome, are poorly understood. This project will apply next generation sequencing technology to chart the occurrence of m5C in eukaryotic cellular RNAs and endeavour to unravel its function(s) in human biology and cancer.

修饰的碱基5-甲基胞嘧啶(M5C)在DNA中作为表观遗传标记的作用得到了广泛的重视和深入的研究。相比之下，RNA分子中相同碱基修饰的细胞功能--充当DNA基因组的工作副本--却鲜为人知。本项目将应用下一代测序技术绘制M5C在真核细胞RNA中的出现情况，并努力揭示其在人类生物学和癌症中的功能(S)。