Analytical epifluorescence microscope

分析型落射荧光显微镜

• 批准号: 359917-2008
• 负责人: Kaminskyj, Susan
• 金额: $ 9.11万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=363766
• 关键词: Analytical; epifluorescence; microscope

Our request for an Analytical Epifluorescence microscope will substantially increase the productivity of several groups in two units at the University of Saskatchewan, Biology and Anatomy/Cell Biology. This system will provide increased access to and enhanced capability for high resolution fluorescence imaging and analysis. This is essential for publication in high-impact journals. We use two epifluorescence systems, a confocal laser scanning microscope (CLSM) purchased in 2002, and upgraded for three additional types of analysis in 2005, and a basic epifluorescence microscope, purchased in 1989. Both are heavily used. Limited time on these instruments has sometimes adversely affected research progress by members of these groups. Need for fluorescence imaging is increasing as molecular genetic methods improve.  Each of the proposed systems produces high-resolution fluorescence images, with multiple focal level reconstruction and time-lapse imaging. Each costs a fraction of the purchase and operational costs of an entry-level CLSM. The lasers in a CLSM are expensive and have limited lifetimes: we have already replaced the $12k argon laser twice. Each of the proposed systems has a wider range of excitation wavelengths than is available with our CLSM, especially at wavelengths that are optimal for certain cell wall and DNA stains. The new system will be mounted on an upright microscope, to complement the inverted microscope on the CLSM, and will have a heated stage, which we currently lack. Access to high-resolution imaging with the addition of a third fluorescence system will allow for better use of the specialized features on the CLSM, that do not need to be duplicated. The upright format and heated stage will permit studies that currently are not possible.    In sum, purchase of a new Analytical Epifluorescence Microscope will facilitate increased research intensity in several groups at the University of Saskatchewan, by providing both enhanced access to new types of fluorescence imaging, and the ability to undertake temperature analyses. Consistent with this, our proposal has attracted matching funds from research units and individual faculty.

我们对分析荧光显微镜的要求将大大提高萨斯喀彻温大学生物学和解剖学/细胞生物学两个单位中几个小组的生产力。这一系统将增加获得高分辨率荧光成像和分析的机会，并增强其能力。这对于在高影响力期刊上发表论文至关重要。我们使用两个落射荧光系统，共聚焦激光扫描显微镜（CLSM）在2002年购买，并在2005年升级为三个额外类型的分析，和一个基本的落射荧光显微镜，在1989年购买。两者都被大量使用。研究这些文书的时间有限，有时对这些小组成员的研究进展产生不利影响。随着分子遗传学方法的改进，对荧光成像的需求也在增加。每一种提出的系统都能产生高分辨率的荧光图像，具有多焦点水平重建和延时成像。每一个成本都是入门级CLSM的购买和运营成本的一小部分。CLSM中的激光器价格昂贵，寿命有限：我们已经两次更换了1.2万美元的氩激光器。每一个提出的系统具有更宽的激发波长范围比我们的CLSM可用，特别是在某些细胞壁和DNA染色的最佳波长。新系统将安装在一个直立显微镜上，以补充CLSM上的倒置显微镜，并将有一个加热的阶段，这是我们目前缺乏的。通过增加第三个荧光系统获得高分辨率成像，将允许更好地使用CLSM上的专用功能，这些功能不需要复制。直立格式和加热阶段将允许目前不可能的研究。 总之，购买新的分析落射荧光显微镜将有助于提高萨斯喀彻温大学几个小组的研究强度，通过提供增强的新型荧光成像和进行温度分析的能力。与此相一致，我们的建议吸引了来自研究单位和个别教师的配套资金。