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A flow cytometry facility to study brain processes and neuro-immune interactions

用于研究大脑过程和神经免疫相互作用的流式细胞术设备

基本信息

批准号：
390349-2010
负责人：
Cermakian, Nicolas
金额：
$ 10.35万
依托单位：
McGill University
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2009
资助国家：
加拿大
起止时间：
2009-01-01 至 2010-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443170
关键词：
flow cytometry facility study brain

项目摘要

This application is to request funding for a flow cytometer. Flow cytometry is the method of choice for a variety of measures to be done in heterogenous cell populations and complex samples, because it allows identifying characteristics and identity at the single-cell level. We wish to apply this technique to various aspects of our research programs. Research aimed at deciphering brain function, and cross-talk between the brain and other physiological systems (e.g. the immune system), is critical for understanding mental and neurological disorders. Since highly complex processes are involved, accurate and efficient ways of monitoring cell functions are needed. In particular, one needs to assess gene expression and cell composition in different tissues under various pathological conditions, as well as in response to drugs and infection. The research performed using the flow cytometer requested in this application will lead to a better understanding of how brain works, including biological timing, learning and memory, brain development, and mechanisms of psychiatric disorders. Another benefit of acquiring this equipment will be improved training of graduate students and postdoctoral fellows: the use of the flow cytometry technology will constitute an important part of their diversified training in neuroscience.

此申请是为了申请流式细胞仪的资金。流式细胞术是在异源细胞群和复杂样品中进行各种测量的首选方法，因为它允许在单细胞水平上识别特征和身份。我们希望将这种技术应用于我们研究计划的各个方面。旨在破译大脑功能以及大脑与其他生理系统（例如免疫系统）之间的串扰的研究对于理解精神和神经系统疾病至关重要。由于涉及高度复杂的过程，因此需要准确和有效的方法来监测细胞功能。特别是，人们需要评估在各种病理条件下以及对药物和感染的反应中不同组织中的基因表达和细胞组成。使用本申请中要求的流式细胞仪进行的研究将有助于更好地了解大脑如何工作，包括生物计时，学习和记忆，大脑发育以及精神疾病的机制。获得这一设备的另一个好处将是改善对研究生和博士后研究员的培训：流式细胞术技术的使用将构成他们在神经科学方面的多样化培训的重要组成部分。