Cryo-ultramicrotome for Bioimaging

UBC生物成像设施(BIF)是一个具有强大培训任务的显微镜资源设施。我们需要一台冷冻超微切割机，以改善显微镜下的生物样品制备以及对UBC学生和教职员工的培训。冷冻超微切割机专门用于切割极薄的冰冻生物材料切片，但它的用途也很广泛，因为它也可以用于常规制备的标本。对于光学显微镜和透射电子显微镜的许多类型的样品制备都需要它。BIF既是研究人员将他们的项目带到这里让我们的技术人员为他们执行工作的服务设施，也是我们的技术人员培训研究人员使用设备进行自己研究的资源设施。BIF拥有最新的光学显微镜和透射电子显微镜技术，在样品制备方面遇到了瓶颈。我们目前有一台超微切割机。一种新的冷冻超微切割机将允许我们将目前的服务扩展到更多的研究人员，并允许我们培训更多的学生和教师进行他们自己的研究。此外，一种新的冷冻超微切割机将扩大我们目前在不断增长的冷冻生物样品成像领域的专业知识。这些样品被保存在其原始状态，不受化学固定剂的影响，因此这些样品是无人工制品的。BIF目前拥有一些最新的冰冻样本成像技术，BIF的工作人员以其在该领域的专业知识而闻名国际。BIF中的一个新的冷冻超微切割机将加强和提高我们的专业知识，并增加UBC的研究产出。

The UBC Bioimaging Facility (BIF) is a microscopy resource facility with a strong training mandate. We require a cryo-ultramicrotome to improve both biological sample preparation for microscopy and the training of students and faculty at UBC. A cryo-ultramicrotome is specialized for cutting extremely thin slices of frozen biological material but is versatile in that it may also be used for conventionally prepared specimens. It is required for many types of sample preparation for both light microscopy and transmission electron microscopy. The BIF serves as both a service facility to which researchers bring their projects to have our technicians perform the work for them, and a resource facility where researchers come to be trained by our technicians to use the equipment to perform their own research. BIF houses the latest light microscope and transmission electron microscope technology and is experiencing a bottleneck with respect to sample preparation. We currently have one ultramicrotome. A new cryo-ultramicrotome will allow us to expand our current service to more researchers and to allow us to train more students and faculty to perform their own research. As well, a new cryo-ultramicrotome will expand our current expertise with respect to the growing field of imaging frozen biological samples. These samples are preserved in their native state without being subject to chemical fixatives and therefore the samples are artifact-free. BIF currently houses some of the latest technology for imaging frozen samples and the BIF staff are international known for their expertise in this field. A new cryo-ultramicrotome in the BIF will reinforce and enhance our expertise and augment research output at UBC.